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Title: A new method for studying microaerobic fermentations; 2: An experimental investigation of xylose fermentation

Abstract

A new experimental technique, called oxygen programmed fermentation (OPF), was used to study microbial cultures of the yeasts Pichia stipitis and Candida utilis growing on xylose as carbon and energy source. In the oxygen programmed fermentation, the inlet oxygen mole fraction was continuously changed to scan through a wide range of oxygen uptake rates in a continuous culture. The largest ethanol yields and productivities for P. stipitis were found at oxygen transfer rates below 1.5 mmol L[sup [minus]1] h[sup [minus]1]. It was found that the ratio between the culture fluorescence and near-IR absorbance increased at oxygen transfer rates lower than 1.5 mmol L[sup [minus]1] h[sup [minus]1]. Small amounts of ethanol were produced also by C. utilis when the oxygen transfer rate was between 0 and 3 mmol L[sup [minus]1] h[sup [minus]1]. It is suggested that OPF will form a nice complement to ordinary, microaerobic chemostat experiments, by making the identification of interesting regions of oxygen transfer rates possible in an efficient and time-saving initial experiment.

Authors:
; ;  [1]
  1. Chalmers Univ. of Technology, Goeteborg (Sweden). Dept. of Chemical Reaction
Publication Date:
OSTI Identifier:
7261911
Resource Type:
Journal Article
Journal Name:
Biotechnology and Bioengineering; (United States)
Additional Journal Information:
Journal Volume: 44:4; Journal ID: ISSN 0006-3592
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; ETHANOL; YIELDS; XYLOSE; FERMENTATION; YEASTS; GROWTH; AEROBIC CONDITIONS; CANDIDA; ALCOHOLS; ALDEHYDES; BIOCONVERSION; CARBOHYDRATES; EUMYCOTA; FUNGI; HYDROXY COMPOUNDS; MICROORGANISMS; MONOSACCHARIDES; ORGANIC COMPOUNDS; PENTOSES; PLANTS; SACCHARIDES; 090900* - Biomass Fuels- Processing- (1990-)

Citation Formats

Franzen, C J, Liden, G, and Niklasson, C. A new method for studying microaerobic fermentations; 2: An experimental investigation of xylose fermentation. United States: N. p., 1994. Web. doi:10.1002/bit.260440405.
Franzen, C J, Liden, G, & Niklasson, C. A new method for studying microaerobic fermentations; 2: An experimental investigation of xylose fermentation. United States. doi:10.1002/bit.260440405.
Franzen, C J, Liden, G, and Niklasson, C. Fri . "A new method for studying microaerobic fermentations; 2: An experimental investigation of xylose fermentation". United States. doi:10.1002/bit.260440405.
@article{osti_7261911,
title = {A new method for studying microaerobic fermentations; 2: An experimental investigation of xylose fermentation},
author = {Franzen, C J and Liden, G and Niklasson, C},
abstractNote = {A new experimental technique, called oxygen programmed fermentation (OPF), was used to study microbial cultures of the yeasts Pichia stipitis and Candida utilis growing on xylose as carbon and energy source. In the oxygen programmed fermentation, the inlet oxygen mole fraction was continuously changed to scan through a wide range of oxygen uptake rates in a continuous culture. The largest ethanol yields and productivities for P. stipitis were found at oxygen transfer rates below 1.5 mmol L[sup [minus]1] h[sup [minus]1]. It was found that the ratio between the culture fluorescence and near-IR absorbance increased at oxygen transfer rates lower than 1.5 mmol L[sup [minus]1] h[sup [minus]1]. Small amounts of ethanol were produced also by C. utilis when the oxygen transfer rate was between 0 and 3 mmol L[sup [minus]1] h[sup [minus]1]. It is suggested that OPF will form a nice complement to ordinary, microaerobic chemostat experiments, by making the identification of interesting regions of oxygen transfer rates possible in an efficient and time-saving initial experiment.},
doi = {10.1002/bit.260440405},
journal = {Biotechnology and Bioengineering; (United States)},
issn = {0006-3592},
number = ,
volume = 44:4,
place = {United States},
year = {1994},
month = {8}
}