skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Trypsin-induced ATPase activity in potato mitochondria

Abstract

Potato mitochondria (Solanum tuberosum var. Russet Burbank), which readily phosphorylate ADP in oxidative phosphorylation, show low levels of ATPase activity which is stimulated neither by Mg/sup 2 +/, 2,4-dinitrophenol, incubation with respiratory substrates, nor disruption by sonication or treatment with Triton X-100, individually or in concert. Treatment of disrupted potato mitochondria with trypsin stimulates Mg/sup 2 +/-dependent, oligomycin-sensitive ATPase activity 10- to 15-fold, suggesting the presence of an ATPase inhibitor protein. Trypsin-induced ATPase activity was unaffected by uncoupler. Oligomycin-sensitive ATPase activity decreases as exposure to trypsin is increased. Incubation at alkaline pH or heating at 60/sup 0/C for 2 minutes also activates ATPase of sonicated potato mitochondria. Disruption of cauliflower (Brassica oleracea), red sweet potato (Ipomoea batatas), and carrot (Daucus carota) mitochondria increases ATPase activity, which is further enhanced by treatment with trypsin. The significance of the tight association of the inhibitor protein and ATPase in potato mitochondria is not clear.

Authors:
;
Publication Date:
Research Org.:
Univ. of California, Los Angeles, CA (United States)
OSTI Identifier:
7245659
DOE Contract Number:  
AT(04-3)-34
Resource Type:
Journal Article
Journal Name:
Plant Physiol.; (United States)
Additional Journal Information:
Journal Volume: 57:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ATP-ASE; BIOCHEMICAL REACTION KINETICS; MITOCHONDRIA; TRYPSIN; ADP; ALKALOIDS; ANTIBIOTICS; ATP; BRASSICA; CARROTS; DINITROPHENOL; INHIBITION; MAGNESIUM; PHOSPHORYLATION; POTATOES; PROTEINS; SOUND WAVES; SUBSTRATES; VEGETABLES; ALKALINE EARTH METALS; AROMATICS; BIOMASS; CELL CONSTITUENTS; CHEMICAL REACTIONS; DRUGS; ELEMENTS; ENERGY SOURCES; ENZYMES; ESTERASES; FOOD; HYDROLASES; HYDROXY COMPOUNDS; KINETICS; METALS; NITRO COMPOUNDS; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANOIDS; PEPTIDE HYDROLASES; PHENOLS; PHOSPHATASES; PLANTS; REACTION KINETICS; RENEWABLE ENERGY SOURCES; TUBERS; 550300* - Cytology; 550200 - Biochemistry

Citation Formats

Jung, D W, and Laties, G G. Trypsin-induced ATPase activity in potato mitochondria. United States: N. p., 1976. Web. doi:10.1104/pp.57.4.583.
Jung, D W, & Laties, G G. Trypsin-induced ATPase activity in potato mitochondria. United States. https://doi.org/10.1104/pp.57.4.583
Jung, D W, and Laties, G G. 1976. "Trypsin-induced ATPase activity in potato mitochondria". United States. https://doi.org/10.1104/pp.57.4.583.
@article{osti_7245659,
title = {Trypsin-induced ATPase activity in potato mitochondria},
author = {Jung, D W and Laties, G G},
abstractNote = {Potato mitochondria (Solanum tuberosum var. Russet Burbank), which readily phosphorylate ADP in oxidative phosphorylation, show low levels of ATPase activity which is stimulated neither by Mg/sup 2 +/, 2,4-dinitrophenol, incubation with respiratory substrates, nor disruption by sonication or treatment with Triton X-100, individually or in concert. Treatment of disrupted potato mitochondria with trypsin stimulates Mg/sup 2 +/-dependent, oligomycin-sensitive ATPase activity 10- to 15-fold, suggesting the presence of an ATPase inhibitor protein. Trypsin-induced ATPase activity was unaffected by uncoupler. Oligomycin-sensitive ATPase activity decreases as exposure to trypsin is increased. Incubation at alkaline pH or heating at 60/sup 0/C for 2 minutes also activates ATPase of sonicated potato mitochondria. Disruption of cauliflower (Brassica oleracea), red sweet potato (Ipomoea batatas), and carrot (Daucus carota) mitochondria increases ATPase activity, which is further enhanced by treatment with trypsin. The significance of the tight association of the inhibitor protein and ATPase in potato mitochondria is not clear.},
doi = {10.1104/pp.57.4.583},
url = {https://www.osti.gov/biblio/7245659}, journal = {Plant Physiol.; (United States)},
number = ,
volume = 57:4,
place = {United States},
year = {Thu Apr 01 00:00:00 EST 1976},
month = {Thu Apr 01 00:00:00 EST 1976}
}