Opioid receptors in human neuroblastoma SH-SY5Y cells: evidence for distinct morphine (. mu. ) and enkephalin (delta) binding sites

Journal Article · Fri Jun 13 00:00:00 EDT 1986 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(86)91152-6· OSTI ID:7245141

Kazmi, S M.I.; Mishra, R K

Human neuroblastoma SH-SY5Y cells exhibited a heterogeneous population of ..mu.. and delta types of opioid binding sites. These specific binding sites displayed the characteristic saturability, stereospecificity and reversibility, expected of a receptor. Scatchard analysis of (/sup 3/H)-D-Ala/sup 2/-D-Leu/sup 5/-enkephalin (DADLE) in the presence of 10/sup -5/M D-Pro/sup 4/-morphiceptin (to block the ..mu.. receptors) and the competitive displacement by various highly selective ligands yielded the binding parameters of delta sites which closely resemble those of the delta receptors in brain and mouse neuroblastoma clones. Similarly, the high affinity binding of (/sup 3/H)-dihydromorphine, together with the higher potency of morphine analogues to displace (/sup 3/H)-naloxone binding established the presence of ..mu.. sites. Guanine nucleotides and NaCl significantly inhibited the association and increased the dissociation of (/sup 3/H)-DADLE binding.

• Research Organization: McMaster Univ., Hamilton, Ontario
• OSTI ID: 7245141
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 137:2
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
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BIOCHEMICAL REACTION KINETICS
MORPHINE
TUMOR CELLS
RECEPTORS
DRUGS
EXPERIMENTAL DATA
GUANINE
INHIBITION
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NUCLEOTIDES
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TRITIUM COMPOUNDS
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AMINES
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ANIMAL CELLS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHLORIDES
CHLORINE COMPOUNDS
DATA
ENDORPHINS
HALIDES
HALOGEN COMPOUNDS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MEMBRANE PROTEINS
NARCOTICS
NEUROREGULATORS
NUMERICAL DATA
OPIUM
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PEPTIDES
POLYPEPTIDES
PROTEINS
PURINES
REACTION KINETICS
SODIUM COMPOUNDS
SOMATIC CELLS
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