Increased expression of the epidermal growth factor receptor gene in malignant gliomas is invariably associated with gene amplification
Primary malignant gliomas from 63 patients were analyzed to determine the relationship between amplification of the gene encoding the epidermal growth factor receptor (EGFR) and expression of the corresponding mRNA. Twenty-four tumors were found to have amplified the EGFR gene and amplification of other genes occurred in three additional tumors. Hybridization with synthetic RNA probes was used to quantitate mRNA levels in situ. All 24 tumors with amplification of the EGFR gene had high levels of expression of this gene, while none of the 39 tumors without amplification had increased levels. This shows that, in human gliomas, large increases in the expression of the EGFR gene are invariably associated with alterations in gene structure.
- Research Organization:
- Johns Hopkins Univ. School of Medicine, Baltimore, MD (USA)
- OSTI ID:
- 7241377
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 84:19
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
GLIOMAS
AUTORADIOGRAPHY
MESSENGER-RNA
HYBRIDIZATION
SUBCELLULAR DISTRIBUTION
ONCOGENES
GENE AMPLIFICATION
GENE REGULATION
ELECTROPHORESIS
GROWTH FACTORS
PATIENTS
PHOSPHORUS 32
RECEPTORS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
DISEASES
DISTRIBUTION
GENES
ISOTOPES
LIGHT NUCLEI
MEMBRANE PROTEINS
MITOGENS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
PROTEINS
RADIOISOTOPES
RNA
550601* - Medicine- Unsealed Radionuclides in Diagnostics
550201 - Biochemistry- Tracer Techniques