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Title: Efficient fermentation of Pinus sp. acid hydrolysates by an ethanologenic strain of Escherichia coli

Abstract

Process conditions for the acid hydrolysis of pine hemicellulose and cellulose have been described which provide a biocompatible sugar solution. By using an improved strain of recombinant Escherichia coli, strain KO11, hydrolysates supplemented with yeast extract and tryptone nutrients were converted to ethanol with an efficiency of 85% to over 100% on the basis of monomer sugar content (approximately 72 g/liter) and with the production of 35 g of ethanol per liter in 48 h. In the process described, approximately 347 liters of ethanol could be produced per dry metric ton of lignocellulose.

Authors:
;  [1];  [2]; ;  [3]
  1. Univ. of Florida, Gainesville (United States)
  2. Tennessee Valley Authority, Muscle Shoals, AL (United States)
  3. Bio-hol Developments, Toronto, Ontario (Canada)
Publication Date:
OSTI Identifier:
7239661
Resource Type:
Journal Article
Journal Name:
Applied and Environmental Microbiology; (United States)
Additional Journal Information:
Journal Volume: 58:4; Journal ID: ISSN 0099-2240
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; CELLULOSE; FERMENTATION; ESCHERICHIA COLI; GENETIC ENGINEERING; ETHANOL; BIOSYNTHESIS; ACID HYDROLYSIS; LIGNIN; RECOMBINANT DNA; ALCOHOLS; BACTERIA; BIOCONVERSION; BIOTECHNOLOGY; CARBOHYDRATES; CHEMICAL REACTIONS; DECOMPOSITION; DNA; HYDROLYSIS; HYDROXY COMPOUNDS; LYSIS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; POLYSACCHARIDES; SACCHARIDES; SOLVOLYSIS; SYNTHESIS; 090900* - Biomass Fuels- Processing- (1990-)

Citation Formats

Barbosa, M F.S. de, Ingram, L O, Beck, M J, Fein, J E, and Potts, D. Efficient fermentation of Pinus sp. acid hydrolysates by an ethanologenic strain of Escherichia coli. United States: N. p., 1992. Web.
Barbosa, M F.S. de, Ingram, L O, Beck, M J, Fein, J E, & Potts, D. Efficient fermentation of Pinus sp. acid hydrolysates by an ethanologenic strain of Escherichia coli. United States.
Barbosa, M F.S. de, Ingram, L O, Beck, M J, Fein, J E, and Potts, D. 1992. "Efficient fermentation of Pinus sp. acid hydrolysates by an ethanologenic strain of Escherichia coli". United States.
@article{osti_7239661,
title = {Efficient fermentation of Pinus sp. acid hydrolysates by an ethanologenic strain of Escherichia coli},
author = {Barbosa, M F.S. de and Ingram, L O and Beck, M J and Fein, J E and Potts, D},
abstractNote = {Process conditions for the acid hydrolysis of pine hemicellulose and cellulose have been described which provide a biocompatible sugar solution. By using an improved strain of recombinant Escherichia coli, strain KO11, hydrolysates supplemented with yeast extract and tryptone nutrients were converted to ethanol with an efficiency of 85% to over 100% on the basis of monomer sugar content (approximately 72 g/liter) and with the production of 35 g of ethanol per liter in 48 h. In the process described, approximately 347 liters of ethanol could be produced per dry metric ton of lignocellulose.},
doi = {},
url = {https://www.osti.gov/biblio/7239661}, journal = {Applied and Environmental Microbiology; (United States)},
issn = {0099-2240},
number = ,
volume = 58:4,
place = {United States},
year = {Wed Apr 01 00:00:00 EST 1992},
month = {Wed Apr 01 00:00:00 EST 1992}
}