Rapid assembly of newly synthesized DNA into chromatin subunits prior to joining of small DNA replication intermediates

Journal Article · Mon Nov 08 00:00:00 EST 1976 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(76)90510-6· OSTI ID:7221865

Hildebrand, C E; Walters, R A

Nuclei from cells having the replicating DNA pulse-labeled with (/sup 3/H) thymidine and the nonreplicating DNA uniformly labeled with (/sup 14/C) thymidine were treated with micrococcal nuclease according to procedures which have been used to study the subunit structure of chromatin. Sedimentation analyses of chromatin from nuclease-treated nuclei, together with measurements of the size of newly synthesized DNA, indicate that (1) chromatin subunits near the replication fork are more susceptible to nuclease attack than subunits in nonreplicating chromatin; (2) newly synthesized DNA is rapidly assembled into chromatin subunits prior to joining of small DNA replication intermediates; and (3) within 10 min after synthesis, DNA in newly replicated chromatin acquires a susceptibility to nuclease treatment similar to that of nonreplicating chromatin.

• Research Organization: Los Alamos Scientific Lab., NM
• OSTI ID: 7221865
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 73:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA REPLICATION
ANIMAL CELLS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CELL NUCLEI
CHROMATIN
ENZYMES
HAMSTERS
LABELLING
MICROCOCCUS
MOLECULAR STRUCTURE
NUCLEASES
SEDIMENTATION
THYMIDINE
TRITIUM COMPOUNDS
ANIMALS
AZINES
BACTERIA
CELL CONSTITUENTS
HETEROCYCLIC COMPOUNDS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHOTRANSFERASES
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
RODENTS
SYNTHESIS
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques