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Title: Characterization of a microdissection library from human chromosome region 3p14

Abstract

Structural alterations in human chromosome region 3p14-p23 resulting in the inactivation of one or more tumor suppressor genes are thought to play a pathogenic role in small cell lung cancer, renal cell carcinoma, and other human neoplasms. To identify putative tumor suppressor genes, 428 recombinant clones from a microdissection library specific for human chromosome region 3p14 were isolated and characterized. Ninety-six of these (22.5%) were human single-copy DNA sequences, 57 of which were unique sequence clones. Forty-four of these were mapped to the microdissected region using a cell hybrid mapping panel. Within this mapping panel, four probes detected two new chromosome breakpoints that were previously indistinguishable from the translocation breakpoint t(3;8) in 3p14.2 in hereditary renal cell carcinoma. One probe maps to the homozygously deleted region of the small cell lung cancer cell line U2020. In addition, microdissection clones have been shown to be suitable for isolation of yeast artificial chromosomes. 52 refs., 3 figs., 2 tabs.

Authors:
; ; ;  [1]; ;  [2]; ;  [3]; ;  [4]
  1. (Univ. of Essen Medical School, Essen (Germany))
  2. (Institut fuer Humangenetik, Essen (Germany))
  3. (Institu fuer Humangenetik, Universitaet Erlangen (Germany))
  4. (Wayne State Univ., Detroit, MI (United States)) (and others)
Publication Date:
OSTI Identifier:
7199584
Resource Type:
Journal Article
Resource Relation:
Journal Name: Genomics; (United States); Journal Volume: 19:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; HUMAN CHROMOSOME 3; GENETIC MAPPING; NEOPLASMS; ETIOLOGY; GENES; DNA SEQUENCING; DNA-CLONING; KIDNEYS; BODY; CHROMOSOMES; CLONING; DISEASES; DNA HYBRIDIZATION; HUMAN CHROMOSOMES; HYBRIDIZATION; MAPPING; ORGANS; STRUCTURAL CHEMICAL ANALYSIS; 550400* - Genetics; 550900 - Pathology

Citation Formats

Bardenheuer, W., Szymanski, S., Lux, A., Schuette, J., Luedecke, H.J., Horsthemke, B., Claussen, U., Senger, G., Smith, D.I., and Wang, N.D. Characterization of a microdissection library from human chromosome region 3p14. United States: N. p., 1994. Web. doi:10.1006/geno.1994.1060.
Bardenheuer, W., Szymanski, S., Lux, A., Schuette, J., Luedecke, H.J., Horsthemke, B., Claussen, U., Senger, G., Smith, D.I., & Wang, N.D. Characterization of a microdissection library from human chromosome region 3p14. United States. doi:10.1006/geno.1994.1060.
Bardenheuer, W., Szymanski, S., Lux, A., Schuette, J., Luedecke, H.J., Horsthemke, B., Claussen, U., Senger, G., Smith, D.I., and Wang, N.D. Sat . "Characterization of a microdissection library from human chromosome region 3p14". United States. doi:10.1006/geno.1994.1060.
@article{osti_7199584,
title = {Characterization of a microdissection library from human chromosome region 3p14},
author = {Bardenheuer, W. and Szymanski, S. and Lux, A. and Schuette, J. and Luedecke, H.J. and Horsthemke, B. and Claussen, U. and Senger, G. and Smith, D.I. and Wang, N.D.},
abstractNote = {Structural alterations in human chromosome region 3p14-p23 resulting in the inactivation of one or more tumor suppressor genes are thought to play a pathogenic role in small cell lung cancer, renal cell carcinoma, and other human neoplasms. To identify putative tumor suppressor genes, 428 recombinant clones from a microdissection library specific for human chromosome region 3p14 were isolated and characterized. Ninety-six of these (22.5%) were human single-copy DNA sequences, 57 of which were unique sequence clones. Forty-four of these were mapped to the microdissected region using a cell hybrid mapping panel. Within this mapping panel, four probes detected two new chromosome breakpoints that were previously indistinguishable from the translocation breakpoint t(3;8) in 3p14.2 in hereditary renal cell carcinoma. One probe maps to the homozygously deleted region of the small cell lung cancer cell line U2020. In addition, microdissection clones have been shown to be suitable for isolation of yeast artificial chromosomes. 52 refs., 3 figs., 2 tabs.},
doi = {10.1006/geno.1994.1060},
journal = {Genomics; (United States)},
number = ,
volume = 19:2,
place = {United States},
year = {Sat Jan 15 00:00:00 EST 1994},
month = {Sat Jan 15 00:00:00 EST 1994}
}