Determination of benzo(a)pyrene in the total particulate matter of cigarette smoke

Journal Article · Tue Mar 01 00:00:00 EST 1988 · J. Chromatogr. Sci.; (United States)

DOI:https://doi.org/10.1093/chromsci/26.3.113· OSTI ID:7197365

Risner, C H

A procedure for the isolation and determination of benzo(a)pyrene in the total particulate matter of cigarette smoke is described. Two high-pressure liquid chromatographic (HPLC) techniques are employed: a normal-phase, mu Bondapak-NH2, amino column is used for isolation of the benzo(a)pyrene fraction and a reversed-phase, Vydac 201TP54, polymeric octadecyl silane column is used for quantitation. Fluorescence detection is used in both modes of chromatography. The wavelengths of excitation and emission are evaluated for analytical detection. Extraction media and various isolation techniques are compared for their extraction efficiency and isolation from interferences, respectively. The procedure is efficient, reproducible, sensitive (3 pg), and gives results that compare favorably with other techniques reported in the literature for the B(a)P content of reference cigarettes, 1R1 and 1R4F.

• Research Organization: R.J. Reynolds Tobacco Company, Winston-Salem, NC (USA)
• OSTI ID: 7197365
• Journal Information: J. Chromatogr. Sci.; (United States), Vol. 26:3
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
BENZOPYRENE
LIQUID COLUMN CHROMATOGRAPHY
FLUORESCENCE
PARTICULATES
TOBACCO SMOKES
WAVELENGTHS
AEROSOLS
AROMATICS
CHROMATOGRAPHY
COLLOIDS
CONDENSED AROMATICS
DISPERSIONS
HYDROCARBONS
LUMINESCENCE
ORGANIC COMPOUNDS
PARTICLES
RESIDUES
SEPARATION PROCESSES
SMOKES
SOLS
500200* - Environment
Atmospheric- Chemicals Monitoring & Transport- (-1989)