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Title: A unique enhancer element for the trans activator (p40 sup tax ) of human T-cell leukemia virus type I that is distinct from cyclic AMP- and 12-O-tetradecanoylphobol-13-acetate-responsive elements

Abstract

The trans activator (p40{sup tax}) of human T-cell leukemia virus type I (HTLV-I) is a transcriptional factor that activates the long terminal repeat (LTR) of HTLV-I and interleukin-2 receptor {alpha}. The authors examined the HTLV-I enhancer responsible for tax-mediated trans activation and identified (A/T)(G/C)(G/C)CNNTGACG(T/A) as a plausible tax-responsive element (TRE). The putative TRE in the LTR was found to be different from the elements required for activation by cyclic AMP and 12-O-tetradecanoylphorbol-13-acetate, although these elements overlapped each other. The TRE was also different from a binding site of N-{kappa}B-like factor that was identified was identified in the interleukin-2 receptor {alpha} promoter and human immunodeficiency virus LTR as a TRE. The latter result was further demonstrated by the failure of the NF-{kappa}B sequence to compete with the TRE of the LTR in a protein-binding assay. These findings indicate that tax function and its cascade can modulate activities of various enhancer sequences, which are probably regulated by distinct DNA-binding factors.

Authors:
; ;  [1]
  1. Cancer Institute, Tokyo (Japan)
Publication Date:
OSTI Identifier:
7195040
Resource Type:
Journal Article
Journal Name:
Journal of Virology; (USA)
Additional Journal Information:
Journal Volume: 63:8; Journal ID: ISSN 0022-538X
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AMP; BIOLOGICAL EFFECTS; LEUKEMIA VIRUSES; CHEMICAL ACTIVATION; PHORBOL ESTERS; CHLORAMPHENICOL; DNA; DNA SEQUENCING; ENZYME ACTIVITY; LYMPHOKINES; PHOSPHORUS 32; PLASMIDS; RECEPTORS; TRANSFERASES; ANTI-INFECTIVE AGENTS; ANTIBIOTICS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARCINOGENS; CELL CONSTITUENTS; DAYS LIVING RADIOISOTOPES; DRUGS; ENZYMES; ESTERS; GROWTH FACTORS; ISOTOPES; LIGHT NUCLEI; MEMBRANE PROTEINS; MICROORGANISMS; MITOGENS; NUCLEI; NUCLEIC ACIDS; NUCLEOTIDES; ODD-ODD NUCLEI; ONCOGENIC VIRUSES; ORGANIC COMPOUNDS; PARASITES; PHOSPHORUS ISOTOPES; PROTEINS; RADIOISOTOPES; STRUCTURAL CHEMICAL ANALYSIS; VIRUSES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Fujisawa, Junichi, Toita, Masami, and Yoshida, Mitsuaki. A unique enhancer element for the trans activator (p40 sup tax ) of human T-cell leukemia virus type I that is distinct from cyclic AMP- and 12-O-tetradecanoylphobol-13-acetate-responsive elements. United States: N. p., 1989. Web.
Fujisawa, Junichi, Toita, Masami, & Yoshida, Mitsuaki. A unique enhancer element for the trans activator (p40 sup tax ) of human T-cell leukemia virus type I that is distinct from cyclic AMP- and 12-O-tetradecanoylphobol-13-acetate-responsive elements. United States.
Fujisawa, Junichi, Toita, Masami, and Yoshida, Mitsuaki. Tue . "A unique enhancer element for the trans activator (p40 sup tax ) of human T-cell leukemia virus type I that is distinct from cyclic AMP- and 12-O-tetradecanoylphobol-13-acetate-responsive elements". United States.
@article{osti_7195040,
title = {A unique enhancer element for the trans activator (p40 sup tax ) of human T-cell leukemia virus type I that is distinct from cyclic AMP- and 12-O-tetradecanoylphobol-13-acetate-responsive elements},
author = {Fujisawa, Junichi and Toita, Masami and Yoshida, Mitsuaki},
abstractNote = {The trans activator (p40{sup tax}) of human T-cell leukemia virus type I (HTLV-I) is a transcriptional factor that activates the long terminal repeat (LTR) of HTLV-I and interleukin-2 receptor {alpha}. The authors examined the HTLV-I enhancer responsible for tax-mediated trans activation and identified (A/T)(G/C)(G/C)CNNTGACG(T/A) as a plausible tax-responsive element (TRE). The putative TRE in the LTR was found to be different from the elements required for activation by cyclic AMP and 12-O-tetradecanoylphorbol-13-acetate, although these elements overlapped each other. The TRE was also different from a binding site of N-{kappa}B-like factor that was identified was identified in the interleukin-2 receptor {alpha} promoter and human immunodeficiency virus LTR as a TRE. The latter result was further demonstrated by the failure of the NF-{kappa}B sequence to compete with the TRE of the LTR in a protein-binding assay. These findings indicate that tax function and its cascade can modulate activities of various enhancer sequences, which are probably regulated by distinct DNA-binding factors.},
doi = {},
journal = {Journal of Virology; (USA)},
issn = {0022-538X},
number = ,
volume = 63:8,
place = {United States},
year = {1989},
month = {8}
}