Siderophore-mediated uptake of Fe sup 3+ by the plant growth-stimulating Pseudomonas putida strain WCS358 and by other rhizosphere microorganisms

Journal Article · Sat Oct 01 00:00:00 EDT 1988 · Journal of Bacteriology; (USA)

OSTI ID:7194957

de Weger, L A; van Arendonk, J J.C.M.; Recourt, K; Lugtenberg, B ^[1]; van der Hofstad, G A.J.M.; Weisbeek, P J ^[2]

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1. Leiden Univ. (Netherlands)
2. Univ. of Utrecht (Netherlands)

Under iron-limited conditions, Pseudomonas putida WCS358 produces a siderophore, pseudobactin 358, which is essential for the plant growth-stimulating ability of this strain. Cells of strain WCS358, provided that they have been grown under Fe{sup 3+} limitation, take up {sup 55}Fe{sup 3+} from the {sup 55}Fe{sup 3+}-labeled pseudobactin 358 complex with K{sub m} and V{sub max} values of 0.23 {mu}M and 0.14 nmol/mg of cell dry weight per min, respectively. Uptake experiments with cells treated with various metabolic inhibitors showed that this Fe{sup 3+} uptake process was dependent on the proton motive force. Furthermore, strain WCS358 was shown to be able to take up Fe{sup 3+} complexed to the siderophore of another plant-beneficial P. fluorescens strain, WCS374. The tested pathogenic rhizobacteria and rhizofungi were neither able to grow on Fe{sup 3+}-deficient medium in the presence of pseudobactin 358 nor able to take up {sup 55}Fe{sub 3+} from {sup 55}Fe{sup 3+}-pseudobactin 358. The same applies for three cyanide-producing Pseudomonas strains which are supposed to be representatives of the minor pathogens. These results indicate that the extraordinary ability of strain WCS358 to compete efficiently for Fe{sup 3+} is based on the fact that the pathogenic and deleterious rhizosphere microorganisms, in contrast to strain WCS358 itself, are not able to take up Fe{sup 3+} from Fe{sup 3+}-pseudobactin 358 complexes.

• OSTI ID: 7194957
• Journal Information: Journal of Bacteriology; (USA), Vol. 170:10; ISSN 0021-9193
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
IRON COMPOUNDS
UPTAKE
PSEUDOMONAS
METABOLISM
FUNGI
IRON 55
LABELLED COMPOUNDS
PATHOGENS
PESTICIDES
PLANT GROWTH
STIMULATION
TRACER TECHNIQUES
BACTERIA
BETA DECAY RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
GROWTH
INTERMEDIATE MASS NUCLEI
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MICROORGANISMS
NUCLEI
PLANTS
RADIOISOTOPES
TRANSITION ELEMENT COMPOUNDS
YEARS LIVING RADIOISOTOPES
550501* - Metabolism- Tracer Techniques