Effect of deoxyribonucleic acid replication inhibitors on bacterial recombination. [UV radiation, nalidixic acid, 6-(p-hydroxyphenylazo)-uracil]
Abstract
Two inhibitors of replicative deoxyribonucleic acid (DNA) synthesis, nalidixic acid (NAL) and 6-(p-hydroxyphenylazo)-uracil (HPUra), showed different effects on genetic recombination and DNA repair in Bacillus subtilis. Previous work (Pedrini et al., 1972) showed that NAL does not interfere with the transformation process of B. subtilis. The results reported in this work demonstrated that the drug was also without effect on the transfection SPP1 or SPO-1 phage DNA (a process that requires a recombination event). The drug was also ineffective on the host cell reactivation of ultraviolet-irradiated SPP1 phage, as well as on transfection with ultraviolet-irradiated DNA of the same phage. HPUra instead markedly reduced the transformation process, as well as transfection, by SPO-1 DNA, but it did not affect the host cell reactivation of SPO-1 phage. In conclusion, whereas the NAL target seems to be specific for replicative DNA synthesis, the HPUra target (i.e., the DNA polymerase III of B. subtilis) seems to be involved also in recombination, but not in the excision repair process. The mutations conferring NAL and HPUra resistance used in this work were mapped by PBS-1 transduction.
- Authors:
- Publication Date:
- Research Org.:
- Univ., Pavia
- OSTI Identifier:
- 7193031
- Resource Type:
- Journal Article
- Journal Name:
- J. Bacteriol.; (United States)
- Additional Journal Information:
- Journal Volume: 126:1
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BACILLUS SUBTILIS; BIOLOGICAL RADIATION EFFECTS; GENE RECOMBINATION; BACTERIOPHAGES; DNA; INHIBITION; ULTRAVIOLET RADIATION; URACILS; AZINES; BACILLUS; BACTERIA; BIOLOGICAL EFFECTS; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PARASITES; PYRIMIDINES; RADIATION EFFECTS; RADIATIONS; VIRUSES; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
Citation Formats
Canosi, U, Siccardi, A G, Falaschi, A, and Mazza, G. Effect of deoxyribonucleic acid replication inhibitors on bacterial recombination. [UV radiation, nalidixic acid, 6-(p-hydroxyphenylazo)-uracil]. United States: N. p., 1976.
Web.
Canosi, U, Siccardi, A G, Falaschi, A, & Mazza, G. Effect of deoxyribonucleic acid replication inhibitors on bacterial recombination. [UV radiation, nalidixic acid, 6-(p-hydroxyphenylazo)-uracil]. United States.
Canosi, U, Siccardi, A G, Falaschi, A, and Mazza, G. 1976.
"Effect of deoxyribonucleic acid replication inhibitors on bacterial recombination. [UV radiation, nalidixic acid, 6-(p-hydroxyphenylazo)-uracil]". United States.
@article{osti_7193031,
title = {Effect of deoxyribonucleic acid replication inhibitors on bacterial recombination. [UV radiation, nalidixic acid, 6-(p-hydroxyphenylazo)-uracil]},
author = {Canosi, U and Siccardi, A G and Falaschi, A and Mazza, G},
abstractNote = {Two inhibitors of replicative deoxyribonucleic acid (DNA) synthesis, nalidixic acid (NAL) and 6-(p-hydroxyphenylazo)-uracil (HPUra), showed different effects on genetic recombination and DNA repair in Bacillus subtilis. Previous work (Pedrini et al., 1972) showed that NAL does not interfere with the transformation process of B. subtilis. The results reported in this work demonstrated that the drug was also without effect on the transfection SPP1 or SPO-1 phage DNA (a process that requires a recombination event). The drug was also ineffective on the host cell reactivation of ultraviolet-irradiated SPP1 phage, as well as on transfection with ultraviolet-irradiated DNA of the same phage. HPUra instead markedly reduced the transformation process, as well as transfection, by SPO-1 DNA, but it did not affect the host cell reactivation of SPO-1 phage. In conclusion, whereas the NAL target seems to be specific for replicative DNA synthesis, the HPUra target (i.e., the DNA polymerase III of B. subtilis) seems to be involved also in recombination, but not in the excision repair process. The mutations conferring NAL and HPUra resistance used in this work were mapped by PBS-1 transduction.},
doi = {},
url = {https://www.osti.gov/biblio/7193031},
journal = {J. Bacteriol.; (United States)},
number = ,
volume = 126:1,
place = {United States},
year = {Thu Apr 01 00:00:00 EST 1976},
month = {Thu Apr 01 00:00:00 EST 1976}
}