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Title: Differential alterations of cortical glutamatergic binding sites in senile dementia of the Alzheimer type

Abstract

Involvement of cortical glutamatergic mechanisms in senile dementia of the Alzheimer type (SDAT) has been investigated with quantitative ligand-binding autoradiography. The distribution and density of Na(+)-dependent glutamate uptake sites and glutamate receptor subtypes--kainate, quisqualate, and N-methyl-D-aspartate--were measured in adjacent sections of frontal cortex obtained postmortem from six patients with SDAT and six age-matched controls. The number of senile plaques was determined in the same brain region. Binding of D-(3H)aspartate to Na(+)-dependent uptake sites was reduced by approximately 40% throughout SDAT frontal cortex relative to controls, indicating a general loss of glutamatergic presynaptic terminals. (3H)Kainate receptor binding was significantly increased by approximately 70% in deep layers of SDAT frontal cortex compared with controls, whereas this binding was unaltered in superficial laminae. There was a positive correlation (r = 0.914) between kainate binding and senile plaque number in deep cortical layers. Quisqualate receptors, as assessed by 2-amino-3-hydroxy-5-(3H)methylisoxazole-4-propionic acid binding, were unaltered in SDAT frontal cortex compared with controls. There was a small reduction (25%) in N-methyl-D-aspartate-sensitive (3H)glutamate binding only in superficial cortical layers of SDAT brains relative to control subjects. (3H)Glutamate binding in SDAT subjects was unrelated to senile plaque number in superficial cortical layers (r = 0.104). These results indicate thatmore » in the presence of cortical glutamatergic terminal loss in SDAT plastic alterations occur in some glutamate receptor subtypes but not in others.« less

Authors:
; ; ; ;  [1]
  1. (Univ. of Glasgow, Scotland (England))
Publication Date:
OSTI Identifier:
7190538
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proceedings of the National Academy of Sciences of the United States of America; (USA); Journal Volume: 87:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GLUTAMIC ACID; RECEPTORS; NERVOUS SYSTEM DISEASES; PATHOGENESIS; BIOCHEMICAL REACTION KINETICS; ASPARTIC ACID; AUTORADIOGRAPHY; CEREBRAL CORTEX; PATIENTS; TRITIUM COMPOUNDS; AMINO ACIDS; BODY; BRAIN; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; CEREBRUM; DISEASES; HYDROGEN COMPOUNDS; KINETICS; MEMBRANE PROTEINS; NERVOUS SYSTEM; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; REACTION KINETICS; 550901* - Pathology- Tracer Techniques

Citation Formats

Chalmers, D.T., Dewar, D., Graham, D.I., Brooks, D.N., and McCulloch, J. Differential alterations of cortical glutamatergic binding sites in senile dementia of the Alzheimer type. United States: N. p., 1990. Web. doi:10.1073/pnas.87.4.1352.
Chalmers, D.T., Dewar, D., Graham, D.I., Brooks, D.N., & McCulloch, J. Differential alterations of cortical glutamatergic binding sites in senile dementia of the Alzheimer type. United States. doi:10.1073/pnas.87.4.1352.
Chalmers, D.T., Dewar, D., Graham, D.I., Brooks, D.N., and McCulloch, J. Thu . "Differential alterations of cortical glutamatergic binding sites in senile dementia of the Alzheimer type". United States. doi:10.1073/pnas.87.4.1352.
@article{osti_7190538,
title = {Differential alterations of cortical glutamatergic binding sites in senile dementia of the Alzheimer type},
author = {Chalmers, D.T. and Dewar, D. and Graham, D.I. and Brooks, D.N. and McCulloch, J.},
abstractNote = {Involvement of cortical glutamatergic mechanisms in senile dementia of the Alzheimer type (SDAT) has been investigated with quantitative ligand-binding autoradiography. The distribution and density of Na(+)-dependent glutamate uptake sites and glutamate receptor subtypes--kainate, quisqualate, and N-methyl-D-aspartate--were measured in adjacent sections of frontal cortex obtained postmortem from six patients with SDAT and six age-matched controls. The number of senile plaques was determined in the same brain region. Binding of D-(3H)aspartate to Na(+)-dependent uptake sites was reduced by approximately 40% throughout SDAT frontal cortex relative to controls, indicating a general loss of glutamatergic presynaptic terminals. (3H)Kainate receptor binding was significantly increased by approximately 70% in deep layers of SDAT frontal cortex compared with controls, whereas this binding was unaltered in superficial laminae. There was a positive correlation (r = 0.914) between kainate binding and senile plaque number in deep cortical layers. Quisqualate receptors, as assessed by 2-amino-3-hydroxy-5-(3H)methylisoxazole-4-propionic acid binding, were unaltered in SDAT frontal cortex compared with controls. There was a small reduction (25%) in N-methyl-D-aspartate-sensitive (3H)glutamate binding only in superficial cortical layers of SDAT brains relative to control subjects. (3H)Glutamate binding in SDAT subjects was unrelated to senile plaque number in superficial cortical layers (r = 0.104). These results indicate that in the presence of cortical glutamatergic terminal loss in SDAT plastic alterations occur in some glutamate receptor subtypes but not in others.},
doi = {10.1073/pnas.87.4.1352},
journal = {Proceedings of the National Academy of Sciences of the United States of America; (USA)},
number = ,
volume = 87:4,
place = {United States},
year = {Thu Feb 01 00:00:00 EST 1990},
month = {Thu Feb 01 00:00:00 EST 1990}
}