Characterization of inositol phosphates in carrot (Daucus carota L. ) cells

Journal Article · Sun Jan 01 00:00:00 EST 1989 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.89.1.126· OSTI ID:7190395

Rincon, M; Chen, Q; Boss, W F ^[1]

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1. North Carolina State Univ., Raleigh (USA)

We have shown previously that inositol-1,4,5-trisphosphate (IP{sub 3}) stimulates an efflux of {sup 45}Ca{sup 2+} from fusogenic carrot protoplasts. In light of these results, we suggested that IP{sub 3} might serve as a second messenger for the mobilization of intracellular Ca{sup 2+} in higher plant cells. To determine whether or not IP{sub 3} and other inositol phosphates were present in the carrot cells, the cells were labeled with myo-(2-{sup 3}H)inositol for 18 hours and extracted with ice-cold 10% trichloroacetic acid. The inositol metabolites were separated by anion exchange chromatography and by paper electrophoresis. We found that ({sup 3}H)inositol metabolites coeluted with inositol bisphosphate (IP{sub 2}) and IP{sub 3} when separated by anion exchange chromatography. However, we could not detect IP{sub 2} or IP{sub 3} when the inositol metabolites were analyzed by paper electrophoresis even though the polyphosphoinositides, which are the source of IP{sub 2} and IP{sub 3}, were present in these cells. Thus, ({sup 3}H)inositol metabolites other than IP{sub 2} and IP{sub 3} had coeluted on the anion exchange columns. The data indicate that either IP{sub 3} is rapidly metabolized or that it is not present at a detectable level in the carrot cells.

• DOE Contract Number: FG05-87ER13693
• OSTI ID: 7190395
• Journal Information: Plant Physiology; (USA), Vol. 89:1; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CALCIUM COMPOUNDS
BIOCHEMICAL REACTION KINETICS
INOSITOL
PLANT CELLS
PHYSIOLOGY
CARROTS
METABOLISM
METABOLITES
PHOSPHATES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
CARBOHYDRATES
FOOD
HYDROGEN COMPOUNDS
INOSITOLS
ISOTOPE APPLICATIONS
KINETICS
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PLANTS
REACTION KINETICS
SACCHARIDES
VEGETABLES
551001* - Physiological Systems- Tracer Techniques