Immunoassay of human TSH using dried blood samples. [/sup 125/I]
A sensitive, semi-quantitative radioimmunoassay method to screen for elevations of TSH concentration in blood is described. The method requires two 0.32 cm dots of dried blood-impregnated filter paper (equivalent to 3 ..mu..l plasma) and a 3-day incubation. Separation of bound and free is obtained using polyethylene glycol. The method can recognize TSH concentrations as low as 22 ..mu..U/ml using a highly sensitive antiserum developed by one of us (AFP). TSH in dried cord and newborn blood from four infants with congenital hypothyroidism was clearly higher than in normal infants. The method is suitable for use in a newborn screening program to confirm the suspicion of primary hypothyroidism in specimens with low T/sub 4/ concentrations.
- Research Organization:
- Peter Bent Brigham Hospital, Boston
- OSTI ID:
- 7190314
- Journal Information:
- J. Clin. Endocrinol. Metab.; (United States), Vol. 42:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
TSH
RADIOIMMUNOASSAY
BLOOD
IMMUNE SERUMS
INFANTS
IODINE 125
LABELLED COMPOUNDS
PATIENTS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY FLUIDS
CHILDREN
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MAN
NEONATES
NUCLEI
ODD-EVEN NUCLEI
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
RADIOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics