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Title: Evidence for effects on the in vivo activity of ribulose-bisphosphate carboxylase/oxygenase during development of Mn toxicity in tobacco. [Nicotiana tabacum L. cv KY14]

Abstract

The progressive decrease in net photosynthesis accompanying development of Mn toxicity in young leaves of burley tobacco (Nicotiana tabacum L. cv KY 14) is a result of effects on in vivo activity of ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (rubisco, EC 4.1.1.39). This conclusion is supported by: (a) decrease in rates of CO{sub 2} depletion during measurements of CO{sub 2} compensation, (b) increase in leaf RuBP concentrations, (c) progressive decreases in rate-constants of RuBP loss (light to dark transition analyses) with progressive increases of leaf Mn concentrations, and (d) restoration of diminished rates of net photosynthesis to control rates by elevated CO{sub 2} (5%). Moreover, elevated CO{sub 2} (1100 microliters per liter) during culture of Mn-treated plants decreased elevated RuBP concentrations to control levels and alleviated foliar symptoms of Mn toxicity. These effects of Mn toxicity on in vivo activity of rubisco were not expressed by in vitro kinetic analyses of rubisco prepared under conditions to sequester Mn or to adsorb polyphenols or their oxidation products. Similarly, the in vitro activity of fructose bisphosphatase (EC 3.1.3.11) was unaffected by Mn toxicity.

Authors:
; ;  [1]
  1. Univ. of Kentucky, Lexington (USA)
Publication Date:
OSTI Identifier:
7189438
Resource Type:
Journal Article
Journal Name:
Plant Physiology; (USA)
Additional Journal Information:
Journal Volume: 86:4; Journal ID: ISSN 0032-0889
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; MANGANESE; TOXICITY; NICOTIANA; PHOTOSYNTHESIS; RIBULOSE DIPHOSPHATE CARBOXYLASE; ENZYME ACTIVITY; BIOCHEMICAL REACTION KINETICS; CARBON DIOXIDE; IN VITRO; IN VIVO; LEAVES; PHOSPHATASES; CARBON COMPOUNDS; CARBON OXIDES; CARBON-CARBON LYASES; CARBOXY-LYASES; CHALCOGENIDES; CHEMICAL REACTIONS; ELEMENTS; ENZYMES; ESTERASES; HYDROLASES; KINETICS; LYASES; MAGNOLIOPHYTA; MAGNOLIOPSIDA; METALS; OXIDES; OXYGEN COMPOUNDS; PHOTOCHEMICAL REACTIONS; PLANTS; REACTION KINETICS; SYNTHESIS; TRANSITION ELEMENTS; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Houtz, R L, Nable, R O, and Cheniae, G M. Evidence for effects on the in vivo activity of ribulose-bisphosphate carboxylase/oxygenase during development of Mn toxicity in tobacco. [Nicotiana tabacum L. cv KY14]. United States: N. p., 1988. Web. doi:10.1104/pp.86.4.1143.
Houtz, R L, Nable, R O, & Cheniae, G M. Evidence for effects on the in vivo activity of ribulose-bisphosphate carboxylase/oxygenase during development of Mn toxicity in tobacco. [Nicotiana tabacum L. cv KY14]. United States. https://doi.org/10.1104/pp.86.4.1143
Houtz, R L, Nable, R O, and Cheniae, G M. Fri . "Evidence for effects on the in vivo activity of ribulose-bisphosphate carboxylase/oxygenase during development of Mn toxicity in tobacco. [Nicotiana tabacum L. cv KY14]". United States. https://doi.org/10.1104/pp.86.4.1143.
@article{osti_7189438,
title = {Evidence for effects on the in vivo activity of ribulose-bisphosphate carboxylase/oxygenase during development of Mn toxicity in tobacco. [Nicotiana tabacum L. cv KY14]},
author = {Houtz, R L and Nable, R O and Cheniae, G M},
abstractNote = {The progressive decrease in net photosynthesis accompanying development of Mn toxicity in young leaves of burley tobacco (Nicotiana tabacum L. cv KY 14) is a result of effects on in vivo activity of ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (rubisco, EC 4.1.1.39). This conclusion is supported by: (a) decrease in rates of CO{sub 2} depletion during measurements of CO{sub 2} compensation, (b) increase in leaf RuBP concentrations, (c) progressive decreases in rate-constants of RuBP loss (light to dark transition analyses) with progressive increases of leaf Mn concentrations, and (d) restoration of diminished rates of net photosynthesis to control rates by elevated CO{sub 2} (5%). Moreover, elevated CO{sub 2} (1100 microliters per liter) during culture of Mn-treated plants decreased elevated RuBP concentrations to control levels and alleviated foliar symptoms of Mn toxicity. These effects of Mn toxicity on in vivo activity of rubisco were not expressed by in vitro kinetic analyses of rubisco prepared under conditions to sequester Mn or to adsorb polyphenols or their oxidation products. Similarly, the in vitro activity of fructose bisphosphatase (EC 3.1.3.11) was unaffected by Mn toxicity.},
doi = {10.1104/pp.86.4.1143},
url = {https://www.osti.gov/biblio/7189438}, journal = {Plant Physiology; (USA)},
issn = {0032-0889},
number = ,
volume = 86:4,
place = {United States},
year = {1988},
month = {4}
}