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Title: Concentration of erythrocyte-based magnetic carriers in the bloodstream

Abstract

The writers postulated that magnetic erythrocytes (ME), injected into the bloodstream, may be concentrated in an assigned region of the vascular bed with the aid of the field of a permanent magnet. To test this hypothesis, erythrocytes ''loaded'' with colloidal magnetite were used, and concentrated in experiments in vitro and in vivo. For the experiments in vivo ME were labeled with sodium pertechnetate (Na /SUP 99n/ TcO4) was estimated in ME with a Rackgamma 1270 counter by determining radioactivity in the ME suspension and in the supernatant. For the experiment in vivo, a midline laparotomy was performed on a dog weighing 14 kg under intravenous hexobarbital anesthesia. The distribution of ME was recorded by means of a gamma-camera.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Institute of Experimental Cardiology, Acad. of Med. Sci.
OSTI Identifier:
7189130
Resource Type:
Journal Article
Resource Relation:
Journal Name: Bull. Exp. Biol. Med. (Engl. Transl.); (United States); Journal Volume: 100:12
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BLOOD VESSELS; MAGNETIC FIELDS; TRACER TECHNIQUES; ERYTHROCYTES; BIOLOGICAL LOCALIZATION; BLOOD CIRCULATION; MAGNETIC MATERIALS; BIOLOGICAL ACCUMULATION; DOGS; IN VITRO; IN VIVO; LABELLED COMPOUNDS; MAGNETIC PROPERTIES; MAGNETITE; SODIUM COMPOUNDS; TECHNETATES; TECHNETIUM 99; ALKALI METAL COMPOUNDS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CARDIOVASCULAR SYSTEM; CHALCOGENIDES; HOURS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IRON COMPOUNDS; IRON ORES; IRON OXIDES; ISOMERIC TRANSITION ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MATERIALS; MINERALS; NUCLEI; ODD-EVEN NUCLEI; ORES; ORGANS; OXIDE MINERALS; OXIDES; OXYGEN COMPOUNDS; PHYSICAL PROPERTIES; RADIOISOTOPES; REFRACTORY METAL COMPOUNDS; TECHNETIUM COMPOUNDS; TECHNETIUM ISOTOPES; TRANSITION ELEMENT COMPOUNDS; VERTEBRATES; YEARS LIVING RADIOISOTOPES 550301* -- Cytology-- Tracer Techniques

Citation Formats

Danilov, Y.N., Il'ina, M.B., Makharudov, S.Y., Orekhov, A.N., Rodchenko, S.A., and Samokhin, G.P.. Concentration of erythrocyte-based magnetic carriers in the bloodstream. United States: N. p., 1986. Web.
Danilov, Y.N., Il'ina, M.B., Makharudov, S.Y., Orekhov, A.N., Rodchenko, S.A., & Samokhin, G.P.. Concentration of erythrocyte-based magnetic carriers in the bloodstream. United States.
Danilov, Y.N., Il'ina, M.B., Makharudov, S.Y., Orekhov, A.N., Rodchenko, S.A., and Samokhin, G.P.. 1986. "Concentration of erythrocyte-based magnetic carriers in the bloodstream". United States. doi:.
@article{osti_7189130,
title = {Concentration of erythrocyte-based magnetic carriers in the bloodstream},
author = {Danilov, Y.N. and Il'ina, M.B. and Makharudov, S.Y. and Orekhov, A.N. and Rodchenko, S.A. and Samokhin, G.P.},
abstractNote = {The writers postulated that magnetic erythrocytes (ME), injected into the bloodstream, may be concentrated in an assigned region of the vascular bed with the aid of the field of a permanent magnet. To test this hypothesis, erythrocytes ''loaded'' with colloidal magnetite were used, and concentrated in experiments in vitro and in vivo. For the experiments in vivo ME were labeled with sodium pertechnetate (Na /SUP 99n/ TcO4) was estimated in ME with a Rackgamma 1270 counter by determining radioactivity in the ME suspension and in the supernatant. For the experiment in vivo, a midline laparotomy was performed on a dog weighing 14 kg under intravenous hexobarbital anesthesia. The distribution of ME was recorded by means of a gamma-camera.},
doi = {},
journal = {Bull. Exp. Biol. Med. (Engl. Transl.); (United States)},
number = ,
volume = 100:12,
place = {United States},
year = 1986,
month = 4
}
  • The method of free paths was used in an analysis of de-Groot type boundary condition in a magnetic field. The boundary conditions are applied in determining the distribution of charged particles along the radius of a cylindrical tube with and without considerations for volume recombination. The calculated curves are correlated with experimental data. (tr-auth)
  • We studied the effect of exercise on blood radiotracer concentration after /sup 99m/Tc in vivo red blood cell labeling. After red blood cell labeling, 13 subjects underwent maximal supine bicycle exercise. Radioactivity, analyzed with a well counter, was measured in heparinized venous blood samples drawn at rest and during peak exercise. Changes in activity were compared with changes in erythrocyte count. Activity and erythrocyte counts increased during exercise in all 13 subjects. Percent increase in activity correlated with percent increase in erythrocyte count (r . -0.78), but did not correlate with either duration of exercise or maximal heart rate. Twentymore » minutes after termination of exercise, activity and erythrocyte count had decreased from peak exercise values but remained higher than preexercise values. In nine nonexercised control subjects, samples drawn 20 minutes apart showed no change in activity or in erythrocyte count. We conclude that exercise increases blood activity, primarily because of an increase in erythrocyte count. During radionuclide ventriculography, blood activity must be measured before and after any intervention, particularly exercise, before a change in left ventricular activity can be attributed to a change in left ventricular volume.« less
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