Simple routine method for enzymatic iodination of peptide hormones

Journal Article · Thu Jan 01 00:00:00 EST 1976 · Anal. Lett.; (United States)

DOI:https://doi.org/10.1080/00032717608059105· OSTI ID:7187965

Schiller, H S; Schlenk, E F; Gosney, K; Chen, W J

A simple enzymatic method for iodinating peptide hormones usable in radioimmunoassays is evaluated. Human LH, human FSH, human and rat prolactin were iodinated multiple times over the past year using similar conditions. Obtained in every instance was an iodinated protein which had little damage and high specific activity and was useful in the respective radioimmunoassay. This method of iodination was recommended for laboratories continually performing multiple peptide hormone radioimmunoassays because of the method's simplicity, consistency and wide versatility. In addition, this method allows radioiodination to desired specific activity. (auth)

• Research Organization: Univ. of Washington, Seattle
• OSTI ID: 7187965
• Journal Information: Anal. Lett.; (United States), Vol. 9:5
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
62 RADIOLOGY AND NUCLEAR MEDICINE
FSH
IODINATION
RADIOIMMUNOASSAY
HORMONES
IODINE 125
CHEMICAL REACTIONS
LH
LTH
PEPTIDES
ANIMAL CELLS
CATALYSIS
CHEMICAL REACTION YIELD
ENZYMES
HYDROGEN PEROXIDE
METABOLISM
RELIABILITY
BETA DECAY RADIOISOTOPES
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GONADOTROPINS
HALOGENATION
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PEROXIDES
PITUITARY HORMONES
PROTEINS
RADIOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
YIELDS
400301* - Organic Chemistry- Chemical & Physicochemical Properties- (-1987)
400702 - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials
550601 - Medicine- Unsealed Radionuclides in Diagnostics