Detection of antibodies to bacterial cell wall peptidoglycan in human sera. [/sup 125/I tracer technique]

Journal Article · Thu Jul 01 00:00:00 EDT 1976 · J. Immunol.; (United States)

OSTI ID:7187499

Heymer, B; Schleifer, K H; Read, S; Zabriskie, J B; Krause, R M

A radioimmunoassay has been developed for the measurement of antibodies to peptidoglycan in human sera including patients with rheumatic feaver and juvenile rheumatoid arthritis. The assay is based on the percentage of binding of the hapten /sup 125/I-L-Ala-..gamma..-D-Glu-L-Lys-D-Ala-D-Ala, the major peptide determinant of peptidoglycan. Because of differences in the avidity of the antibodies in different sera, the amount of antibody was expressed as pentapeptide hapten-binding capacity (pentapeptide-HBC in ng/ml of serum). Fourteen out of 105 normal blood donors had a pentapeptide-HBC value greater than or equal to 75 ng/ml serum. Values in healthy children 5 to 18 years of age were less than or equal to 50 ng/ml. Sixty-eight percent of the individuals with rheumatic fever had values greater than or equal to 75 ng/ml, an indication that streptococcal infections can stimulate an immune response to peptidoglycan. Thirty-five percent of the patients with juvenile rheumatoid arthritis had values greater than or equal to 75 ng/ml. Such a finding points to a possible association between bacterial infections and juvenile rheumatoid arthritis.

• Research Organization: Univ., Ulm, Ger.
• OSTI ID: 7187499
• Journal Information: J. Immunol.; (United States), Vol. 117:1
• Country of Publication: United States
• Language: English

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62 RADIOLOGY AND NUCLEAR MEDICINE
ANTIBODIES
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DIAGNOSIS
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IODINE 125
PATIENTS
TRACER TECHNIQUES
BETA DECAY RADIOISOTOPES
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ODD-EVEN NUCLEI
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