Generalized immunological recognition of the major merozoite surface antigen (gp195) of Plasmodium falciparum

Journal Article · Tue Aug 01 00:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.16.6343· OSTI ID:7168436

Chang, S P; Hui, G S.N.; Kato, A; Siddiqui, W A ^[1]

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1. Univ. of Hawaii, Honolulu (USA)

The antibody response to the Plasmodium falciparum major merozoite surface antigen (gp195) of congenic mouse strains differing in H-2 haplotype has been examined. All seven strains of mice were capable of producing gp195-specific antibodies. Generalized immune recognition of gp195 by mice of diverse H-2 haplotypes distinguished gp195 from the P. falciparum circumsporozoite protein and the 230-kDa and 48/45-kDa gamete surface antigens. However, the H-2 genetic locus appeared to influence the specificity of gp105-specific antibodies. Immunoblot patterns of mouse sera with parasite antigens revealed a complex pattern of reactivity with terminal and intermediate processing fragments of gp195. The majority of immunoblot bands observed were similar for all of the mouse strains; however, there were several strains that additionally recognized a few unique fragments or displayed more intense reactivities with specific processing fragments. These results suggest that while individuals of diverse major histocompatibility complex makeup are capable of recognizing the gp195 antigen, the recognition of specific gp195 B-cell and T-cell epitopes may be under control of the major histocompatibility complex.

• OSTI ID: 7168436
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:16; ISSN 0027-8424
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
HISTOCOMPATIBILITY COMPLEX
GENETICS
MALARIA
VACCINES
MEMBRANE PROTEINS
IMMUNOLOGY
ANTIGENS
BLOOD SERUM
ELECTROPHORESIS
ENZYME IMMUNOASSAY
FLUORESCENCE
MICE
MONOCLONAL ANTIBODIES
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INFECTIOUS DISEASES
INVERTEBRATES
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PARASITES
PARASITIC DISEASES
PROTEINS
PROTOZOA
RODENTS
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550200* - Biochemistry