Tumor necrosis factor induces phosphorylation of a 28-kDa mRNA cap-binding protein in human cervical carcinoma cells

Journal Article · Wed Nov 01 00:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.21.8417· OSTI ID:7165645

Marino, M W; Guidon, Jr, P T; Donner, D B ^[1]; Pfeffer, L M ^[2]

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1. Cornell Univ. Graduate School of Medical Sciences, New York, NY (USA)
2. Rockefeller Univ., New York, NY (USA)

Tumor necrosis factor {alpha} (TNF-{alpha}) stimulated the phosphorylation of a 28-kDa protein (p28) in the ME-180 line of human cervical carcinoma cells. The effect of TNF-{alpha} on the phosphorylation state of p28 was rapid (4-fold increase within 15 min) and persistent, remaining above the basal level for at least 2 hr. The specific binding of {sup 125}I-labeled TNF-{alpha} to cell-surface binding sites, the stimulation of p28 phosphorylation by TNF-{alpha}, and the inhibition of cell proliferation by TNF-{alpha} occurred with nearly identical dose-response relationships. Two-dimensional SDS/PAGE resolved p28 into two isoforms having pI values of 6.2 and 6.1. A phosphorylated cap-binding protein was substantially enriched from lysates of control or TNF-{alpha}-treated ME-180 cells by affinity chromatography with 7-methylguanosine 5{prime}-triposphate-Sepharose. The phosphoprotein recovered from this procedure was the substrate for TNF-{alpha}-promoted phosphorylation, p28. Thus, TNF-{alpha} stimulates the phosphorylation of this mRNA cap-binding protein, which may be involved in the transduction of TNF-{alpha}-receptor binding into cellular responses.

• OSTI ID: 7165645
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:21; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
AUTORADIOGRAPHY
PROTEINS
PHOSPHORYLATION
TUMOR CELLS
CELL PROLIFERATION
CARCINOMAS
ELECTROPHORESIS
IODINE 125
MACROPHAGES
MAN
MESSENGER-RNA
NECROSIS
PHOSPHORUS 32
RECEPTORS
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEMBRANE PROTEINS
MITOGENS
NEOPLASMS
NUCLEI
NUCLEIC ACIDS
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PHAGOCYTES
PHOSPHORUS ISOTOPES
PRIMATES
RADIOISOTOPES
RNA
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques