Differential adhesion of metastatic tumor cells to extracellular matrices of various organs

Conference · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:7164761

Lee, C L; Knudson, W; Coon, J S; Pauli, B U

Malignant tumors metastasize to specific organ sites. This non-random pattern of metastasis may be mediated by components of the extracellular matrix. In this study, they have isolated fibrillar, extracellular matrices from lung, liver, and kidney of rats and mice. Matrices were suspended in MEM medium and placed in 10-ml flasks on a gyrotary shaker or allowed to attach to the bottom of 24-well tissue culture plates during a two hour period. Radio-labeled mouse B16-F10 melanoma cells or rat Mat-LyLu prosatic adenocarcinoma cells, which both metastasize to the lungs, were added to these culture vessels and incubated for 15 or 30 min at 37/sup 0/C. Both cell lines exhibited a preferential adhesion to lung matrix, whereas adhesion to kidney or liver matrices were significantly lower. Mouse and rat tumor cells adhered equally well to the lung matrices prepared from syngeneic or heterogeneic animals. However, when matrices were allowed to dry on the bottom of the dish, the selective adhesion was lost and tumor cells adhered in equal numbers to the three matrices. Extraction of lung matrices with 1 M acetic acid, followed by trypsin digestion of the extracted material indicate that metastasis to the lung is mediated by an acid extractable matrix protein.

• Research Organization: Rush-Presbyterian-St. Luke's Medical Center, Chicago, IL
• OSTI ID: 7164761
• Report Number(s): CONF-8604222-; TRN: 87-003960
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
KIDNEYS
METASTASES
LIVER
LUNGS
TUMOR CELLS
ADHESION
CARCINOMAS
LABELLED COMPOUNDS
MATRICES
MICE
RADIOISOTOPES
RATS
TRACER TECHNIQUES
ANIMAL CELLS
ANIMALS
BODY
DIGESTIVE SYSTEM
DISEASES
GLANDS
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NEOPLASMS
ORGANS
RESPIRATORY SYSTEM
RODENTS
VERTEBRATES
550901* - Pathology- Tracer Techniques