Reverse mapping of the gene encoding the human fos-related antigen-1 (fra-1) within chromosome band 11q13
- University Hospital, Nijmegen (Netherlands)
- Cancer Institute, Tokyo (Japan)
For the identification of sequence-tagged sites within cosmid clones derived from the q13 region of human chromosome 11, the authors subcloned a number of single copy BamHI fragments in a pT7T3 19U vector. Surprisingly, within one of these subclones, they encountered a stretch of DNA that matches perfectly with 5' end of the known cDNA sequence for the human fos-related antigen. This match comes to an abrupt end at nucleotide 328, which coincides with a perfect splice donor site. At position 231 an ATG codon (translation initiation) was found. From these data they conclude that position 328 marks the boundary of the first exon of the fra-1 gene. Homology of this same region with the rat fra-1 cDNA sequence is [+-]70%. This human fra-1 genomic fragment is contained within cosmid clone cCL11-254 (D11S460), which was previously mapped to region V of a long-range contig map of 11q13. The localization of fra-1 on chromosome 11 was cross-checked and confirmed by hybridizing the fra-1 containing fragment to a panel of somatic cell hybrids, including one line that contains chromosome 11 as its only human constituent. In addition to a cross-hybridizing rodent fragment, a single human-specific band was revealed in all the chromosome 11-containing hybrids. This subchromosomal localization of fra-1 to 11q13 adds yet another proto-oncogene to this restricted genomic region, which appears to be involved in a number of neoplastic disorders, e.g., multiple endocrine neoplasia type 1, breast cancer, squamous cell tumors of head and neck, esophageal carcinoma, hepatocellular carcinoma, melanoma, bladder carcinoma, extragonadal germ cell tumors, and some hematopoietic malignancies. 7 refs., 1 fig.
- OSTI ID:
- 7159956
- Journal Information:
- Genomics; (United States), Vol. 18:1; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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