Hydrogen peroxide metabolism in soybean embryonic axes at the onset of germination. [Glycine max]

Journal Article · Mon Feb 01 00:00:00 EST 1988 · Plant Physiology; (USA)

DOI:https://doi.org/10.1104/pp.86.2.626· OSTI ID:7151735

Puntarulo, S; Sanchez, R A; Boveris, A ^[1]

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1. Universidad de Buenos Aires (Argentina)

Hydrogen peroxide steady state levels of 5 micromolar were determined in soybean (Glycine max) embryonic axes incubated for 2 hours and in axes pretreated with aminotriazole or cyanide, where these levels were 50 and 1 micromolar, respectively. The activities of catalase (105 picomoles H{sub 2}O{sub 2} per minute per axis), peroxidase (10-44 picomoles H{sub 2}O{sub 2} per minute per axis), glutathione peroxidase (3 picomoles H{sub 2}O{sub 2} per minute per axis) and superoxide dismutase (3.5 units per axis), were also determined. Catalase seems to be the most important H{sub 2}O{sub 2} consuming enzyme at the physiological concentration of H{sub 2}O{sub 2} level. A short treatment with aminotriazole, while substantially increasing H{sub 2}O{sub 2} level, did not affect the growth of the axes. The production of superoxide anion by the mitochondria isolated from soybean axes was measured from the superoxide dismutase-sensitive rate of adrenochrome formation in the presence of NADH or succinate as substrate and amounted to 1.3 and 0.8 nanomole O{sub 2}{sup {minus}} per minute per milligram protein, respectively. According to the stoichiometry of O{sub 2}{sup {minus}} and H{sub 2}O{sub 2} dismutation reactions, it is apparent that about 0.9 to 1.5% of the total oxygen uptake proceeds through the formation of the free intermediates of the partial reduction of oxygen.

• OSTI ID: 7151735
• Journal Information: Plant Physiology; (USA), Vol. 86:2; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CATALASE
ENZYME ACTIVITY
HYDROGEN PEROXIDE
BIOCHEMICAL REACTION KINETICS
PEROXIDASES
SUPEROXIDE DISMUTASE
ANIONS
CYANIDES
GERMINATION
GLUTATHIONE
GLYCINE HISPIDA
METABOLISM
MITOCHONDRIA
NADH2
OXYGEN
PLANT GROWTH
SEEDS
SUCCINIC ACID
TRIAZOLES
UPTAKE
AZOLES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHARGED PARTICLES
COENZYMES
DICARBOXYLIC ACIDS
DRUGS
ELEMENTS
ENZYMES
GROWTH
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
IONS
KINETICS
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NONMETALS
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANOIDS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEPTIDES
PEROXIDES
PLANTS
POLYPEPTIDES
PROTEINS
RADIOPROTECTIVE SUBSTANCES
REACTION KINETICS
550500* - Metabolism