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Title: RAP30/74: A general initiation factor that binds to RNA polymerase II

Abstract

The authors have previously shown by affinity chromatography that RAP30 and RAP74 are the mammalian proteins that have the highest affinity for RNA polymerase II. Here the authors show that RAP30 binds to RAP74 and that the RAP30-RAP74 complex (RAP30/74) is required for accurate initiation by RNA polymerase II. RAP30/74 is required for accurate transcription from the following promoters: the adenovirus major late promoter, the long terminal repeat of human immunodeficiency virus, P2 of the human c-myc gene, the mouse ..beta../sup maj/-globin promoter (all of which have TATA boxes), and the mouse dihydrofolate reductase promoter (which lacks a TATA box). RAP30/74 is not required for initiation by RNA polymerase III at the adenovirus virus-associated RNA promoters. Therefore, RAP30/74 is a general initiation factor that binds to RNA polymerase II.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Dept. of Biochemistry, Michigan State Univ., East Lansing, MI (US)
OSTI Identifier:
7144004
Resource Type:
Journal Article
Journal Name:
Mol. Cell. Biol.; (United States)
Additional Journal Information:
Journal Volume: 8:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GENE RECOMBINATION PROTEINS; BIOSYNTHESIS; GENE REGULATION; GLOBIN; RNA POLYMERASES; BIOCHEMICAL REACTION KINETICS; ENZYME ACTIVITY; ADENOVIRUS; IMMUNOSUPPRESSION; MICE; OXIDOREDUCTASES; TRANSCRIPTION; ANIMALS; ENZYMES; KINETICS; MAMMALS; MICROORGANISMS; NUCLEOPROTEINS; NUCLEOTIDYLTRANSFERASES; ONCOGENIC VIRUSES; ORGANIC COMPOUNDS; PARASITES; PHOSPHORUS-GROUP TRANSFERASES; POLYMERASES; PROTEINS; REACTION KINETICS; RODENTS; SYNTHESIS; TRANSFERASES; VERTEBRATES; VIRUSES; 550200* - Biochemistry

Citation Formats

Burton, Z.F., Killeen, M., Sopta, M., Ortolan, L.G., and Greenblatt, J. RAP30/74: A general initiation factor that binds to RNA polymerase II. United States: N. p., 1988. Web. doi:10.1128/MCB.8.4.1602.
Burton, Z.F., Killeen, M., Sopta, M., Ortolan, L.G., & Greenblatt, J. RAP30/74: A general initiation factor that binds to RNA polymerase II. United States. doi:10.1128/MCB.8.4.1602.
Burton, Z.F., Killeen, M., Sopta, M., Ortolan, L.G., and Greenblatt, J. Fri . "RAP30/74: A general initiation factor that binds to RNA polymerase II". United States. doi:10.1128/MCB.8.4.1602.
@article{osti_7144004,
title = {RAP30/74: A general initiation factor that binds to RNA polymerase II},
author = {Burton, Z.F. and Killeen, M. and Sopta, M. and Ortolan, L.G. and Greenblatt, J.},
abstractNote = {The authors have previously shown by affinity chromatography that RAP30 and RAP74 are the mammalian proteins that have the highest affinity for RNA polymerase II. Here the authors show that RAP30 binds to RAP74 and that the RAP30-RAP74 complex (RAP30/74) is required for accurate initiation by RNA polymerase II. RAP30/74 is required for accurate transcription from the following promoters: the adenovirus major late promoter, the long terminal repeat of human immunodeficiency virus, P2 of the human c-myc gene, the mouse ..beta../sup maj/-globin promoter (all of which have TATA boxes), and the mouse dihydrofolate reductase promoter (which lacks a TATA box). RAP30/74 is not required for initiation by RNA polymerase III at the adenovirus virus-associated RNA promoters. Therefore, RAP30/74 is a general initiation factor that binds to RNA polymerase II.},
doi = {10.1128/MCB.8.4.1602},
journal = {Mol. Cell. Biol.; (United States)},
number = ,
volume = 8:4,
place = {United States},
year = {1988},
month = {4}
}