Functional characterization of a pyrimidine-rich element in the 5'-noncoding region of the yeast iso-1-cytochrome c gene

Journal Article · Tue Mar 01 00:00:00 EST 1988 · Mol. Cell. Biol.; (United States)

DOI:https://doi.org/10.1128/MCB.8.3.1045· OSTI ID:7143244

McNeil, J B

A predominantly pyrimidine-rich sequence (purine in the template strand, 32 of 37 bases) is located between a functional TATA element and the corresponding transcription start site region of the Saccharomyces cerevisiae iso-1-cytochrome c (CYC1) gene. By using linker deletions and gene fusion techniques, the functional characteristics of this pyrimidine sequence were examined. Results indicate that the function of this element is to limit the accumulation of full-length mRNAs with 5' ends which map upstream of the pyrimidine-rich sequence. Data suggest that the 5'-noncoding region of the CTC1 gene possesses signals for mRNA 3'-end processing.

• Research Organization: Dept. of Microbiology, Univ. of Toronto, Toronto, Ontario (CA)
• OSTI ID: 7143244
• Journal Information: Mol. Cell. Biol.; (United States), Vol. 8:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CYTOCHROMES
GENES
GENETIC MAPPING
PYRIMIDINES
DNA
SACCHAROMYCES CEREVISIAE
CHROMOSOME LOSSES
HYBRIDIZATION
MESSENGER-RNA
RNA PROCESSING
TECHNOLOGY ASSESSMENT
AZINES
FUNGI
HETEROCYCLIC COMPOUNDS
LOSSES
MAPPING
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PLANTS
PROTEINS
RNA
SACCHAROMYCES
YEASTS
550400* - Genetics
550200 - Biochemistry
550700 - Microbiology