Detection of mutant hemoglobins with altered affinity for oxygen

Journal Article · Sat May 01 00:00:00 EDT 1976 · Ann. Intern. Med.; (United States)

DOI:https://doi.org/10.7326/0003-4819-84-5-517· OSTI ID:7143009

Lichtman, M A; Murphy, M S; Adamson, J W

The detection of high- or low-affinity hemoglobins in subjects with polycythemia or anemia is difficult for most physicians because of the requirement for special equipment to do oxygen-hemoglobin dissociation curves. Measurement of the pH, oxygen tension, and oxygen saturation of antecubital venous blood with instruments present in most clinical chemistry laboratories permits an estimate of the strength of oxygen binding to hemoglobin. An equation can be used to convert the venous oxygen tension (standardized to pH 7.4) and the oxygen saturation to the P/sub 50/ of the oxygen-hemoglobin dissociation curve on which the observed point falls. The data indicate that this method is a reliable initial step in the identification of a hemoglobin with abnormal affinity for oxygen and may be applied to population studies, since reliable results are obtained with venous blood stored at 4/sup 0/C for up to 24 hours.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 7143009
• Journal Information: Ann. Intern. Med.; (United States), Vol. 84:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD
CHEMICAL ANALYSIS
HEMOGLOBIN
BINDING ENERGY
MUTANTS
OXYGEN
ANEMIAS
EQUATIONS
MEASURING METHODS
PATIENTS
PH VALUE
POLYCYTHEMIA
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOXYLIC ACIDS
CRYOGENIC FLUIDS
DISEASES
ELEMENTS
ENERGY
FLUIDS
GLOBIN
HEMIC DISEASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
NONMETALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
SYMPTOMS
550200* - Biochemistry