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Title: Strategies for choosing a DNA stain for flow cytometry of metaphase chromosomes

Abstract

Requirements for flow cytometry of metaphase chromosomes stained with three DNA-specific fluorescent dyes (Hoechst 33258, Chromomycin A/sub 3/, and ethidium bromide) are reviewed. Fluorescence properties of these three stains when bound to mitotic cells or to chromosomes in suspension are measured and compared with fluorescence properties when bound to DNA in solution. Conditions are given for high resolution flow cytometry of Chinese hamster chromosomes stained with each of the fluorophors, and histograms are presented which exhibit differences in relative peak position and area. Energy transfer fluorescence between two DNA stains is presented as a potentially useful new parameter for flow cytometry of chromosomes and is illustrated by fluorescence energy transfer from Chromomycin A/sub 3/ to ethidium bromide when simultaneously bound to hamster mitotic cells.

Authors:
; ;
Publication Date:
Research Org.:
California Univ., Livermore (USA). Lawrence Livermore Lab.
OSTI Identifier:
7142989
Report Number(s):
UCRL-79331; CONF-770417-1
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Conference
Resource Relation:
Conference: Symposium on automation and cytochemistry, Chicago, IL, USA, 2 Apr 1977
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANIMAL CELLS; CYTOLOGY; CELL FLOW SYSTEMS; STAINS; DNA; SAMPLE PREPARATION; CELL CYCLE; CHROMOSOMES; DYES; BIOLOGY; NUCLEIC ACIDS; ORGANIC COMPOUNDS; 550300* - Cytology

Citation Formats

Jensen, R H, Langlois, R G, and Mayall, B H. Strategies for choosing a DNA stain for flow cytometry of metaphase chromosomes. United States: N. p., 1977. Web.
Jensen, R H, Langlois, R G, & Mayall, B H. Strategies for choosing a DNA stain for flow cytometry of metaphase chromosomes. United States.
Jensen, R H, Langlois, R G, and Mayall, B H. 1977. "Strategies for choosing a DNA stain for flow cytometry of metaphase chromosomes". United States.
@article{osti_7142989,
title = {Strategies for choosing a DNA stain for flow cytometry of metaphase chromosomes},
author = {Jensen, R H and Langlois, R G and Mayall, B H},
abstractNote = {Requirements for flow cytometry of metaphase chromosomes stained with three DNA-specific fluorescent dyes (Hoechst 33258, Chromomycin A/sub 3/, and ethidium bromide) are reviewed. Fluorescence properties of these three stains when bound to mitotic cells or to chromosomes in suspension are measured and compared with fluorescence properties when bound to DNA in solution. Conditions are given for high resolution flow cytometry of Chinese hamster chromosomes stained with each of the fluorophors, and histograms are presented which exhibit differences in relative peak position and area. Energy transfer fluorescence between two DNA stains is presented as a potentially useful new parameter for flow cytometry of chromosomes and is illustrated by fluorescence energy transfer from Chromomycin A/sub 3/ to ethidium bromide when simultaneously bound to hamster mitotic cells.},
doi = {},
url = {https://www.osti.gov/biblio/7142989}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Apr 04 00:00:00 EST 1977},
month = {Mon Apr 04 00:00:00 EST 1977}
}

Conference:
Other availability
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