skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Rapid metaphase and interphase detection of radiation-induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization

Abstract

Chromosomal in situ suppression (CISS)-hybridization of biotinylated phage DNA-library inserts from sorted human chromosomes was used to decorate chromosomes 1 and 7 specifically from pter to qter and to detect structural aberrations of these chromosomes in irradiated human peripheral lymphocytes. In addition, probe pUC1.77 was used to mark the 1q12 subregion in normal and aberrant chromosomes 1. Low LET radiation (60Co-gamma-rays; 1.17 and 1.33 MeV) of lymphocyte cultures was performed with various doses (D = 0, 2, 4, 8 Gy) 5 h after stimulation with phytohaemagglutinin. Irradiated cells were cultivated for an additional 67 h before Colcemid arrested metaphase spreads were obtained. Aberrations of the specifically stained chromosomes, such as deletions, dicentrics, and rings, were readily scored after in situ hybridization with either the 1q12 specific probe or DNA-library inserts. By the latter approach, translocations of the specifically stained chromosomes could also be reliably assessed. A linear increase of the percentage of specifically stained aberrant chromosomes was observed when plotted as a function of the square of the dose D. A particular advantage of this new approach is provided by the possibility to delineate numerical and structural chromosome aberrations directly in interphase nuclei. These results indicate that cytogenetic monitoring ofmore » ionizing radiation may be considerably facilitated by CISS-hybridization.« less

Authors:
; ; ; ;  [1]
  1. Institut fuer Humangenetik und Anthropolgie, Heidelberg (Germany, F.R.)
Publication Date:
OSTI Identifier:
7136747
Resource Type:
Journal Article
Journal Name:
Cytometry (Baltimore); (USA)
Additional Journal Information:
Journal Volume: 11:1; Journal ID: ISSN 0196-4763
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CHROMOSOMAL ABERRATIONS; RADIOINDUCTION; LYMPHOCYTES; GENETIC RADIATION EFFECTS; CHROMOSOMES; COBALT 60; DNA HYBRIDIZATION; DOSE-RESPONSE RELATIONSHIPS; FLUORESCENCE; GAMMA RADIATION; LET; MAN; MITOSIS; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BIOLOGICAL RADIATION EFFECTS; BLOOD; BLOOD CELLS; BODY FLUIDS; CELL DIVISION; COBALT ISOTOPES; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; ENERGY TRANSFER; GENETIC EFFECTS; HYBRIDIZATION; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LEUKOCYTES; LUMINESCENCE; MAMMALS; MATERIALS; MINUTES LIVING RADIOISOTOPES; MUTATIONS; NUCLEI; ODD-ODD NUCLEI; PRIMATES; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; SOMATIC CELLS; VERTEBRATES; YEARS LIVING RADIOISOT; 560120* - Radiation Effects on Biochemicals, Cells, & Tissue Culture

Citation Formats

Cremer, T, Popp, S, Emmerich, P, Lichter, P, and Cremer, C. Rapid metaphase and interphase detection of radiation-induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization. United States: N. p., 1990. Web. doi:10.1002/cyto.990110113.
Cremer, T, Popp, S, Emmerich, P, Lichter, P, & Cremer, C. Rapid metaphase and interphase detection of radiation-induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization. United States. https://doi.org/10.1002/cyto.990110113
Cremer, T, Popp, S, Emmerich, P, Lichter, P, and Cremer, C. Mon . "Rapid metaphase and interphase detection of radiation-induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization". United States. https://doi.org/10.1002/cyto.990110113.
@article{osti_7136747,
title = {Rapid metaphase and interphase detection of radiation-induced chromosome aberrations in human lymphocytes by chromosomal suppression in situ hybridization},
author = {Cremer, T and Popp, S and Emmerich, P and Lichter, P and Cremer, C},
abstractNote = {Chromosomal in situ suppression (CISS)-hybridization of biotinylated phage DNA-library inserts from sorted human chromosomes was used to decorate chromosomes 1 and 7 specifically from pter to qter and to detect structural aberrations of these chromosomes in irradiated human peripheral lymphocytes. In addition, probe pUC1.77 was used to mark the 1q12 subregion in normal and aberrant chromosomes 1. Low LET radiation (60Co-gamma-rays; 1.17 and 1.33 MeV) of lymphocyte cultures was performed with various doses (D = 0, 2, 4, 8 Gy) 5 h after stimulation with phytohaemagglutinin. Irradiated cells were cultivated for an additional 67 h before Colcemid arrested metaphase spreads were obtained. Aberrations of the specifically stained chromosomes, such as deletions, dicentrics, and rings, were readily scored after in situ hybridization with either the 1q12 specific probe or DNA-library inserts. By the latter approach, translocations of the specifically stained chromosomes could also be reliably assessed. A linear increase of the percentage of specifically stained aberrant chromosomes was observed when plotted as a function of the square of the dose D. A particular advantage of this new approach is provided by the possibility to delineate numerical and structural chromosome aberrations directly in interphase nuclei. These results indicate that cytogenetic monitoring of ionizing radiation may be considerably facilitated by CISS-hybridization.},
doi = {10.1002/cyto.990110113},
url = {https://www.osti.gov/biblio/7136747}, journal = {Cytometry (Baltimore); (USA)},
issn = {0196-4763},
number = ,
volume = 11:1,
place = {United States},
year = {1990},
month = {1}
}