Dual isotope Schilling test for measuring absorption of food-bound and free vitamin B12 simultaneously

Journal Article · Sat Oct 01 00:00:00 EDT 1983 · Am. J. Clin. Pathol.; (United States)

DOI:https://doi.org/10.1093/ajcp/80.4.490· OSTI ID:7133750

Doscherholmen, A; Silvis, S; McMahon, J

A prototype food-bound vitamin B12 (food-B12) absorption test has been developed in which /sup 57/Co-B12 was incorporated in vitro into egg yolk (yolk-B12) and served to volunteers in 50-g cooked portions together with toast and coffee for breakfast. Six hours later, 1 mg nonlabeled B12 was given intramuscularly and 24-hour urine was collected for radioactivity measurement. In separate tests, the absorption of yolk-B12 and crystalline /sup 57/Co-B12 was equally poor in patients with pernicious anemia. However, in patients with simple gastric achlorhydria and those who had undergone gastric surgery, the assimilation of yolk-B12 was impaired greatly, whereas the absorption of crystalline radio-B12 was normal. Egg yolk labeled with /sup 58/Co-B12 was administered together with crystalline /sup 57/Co-B12 in a dual isotope test with results similar to those obtained when the tests were prepared separately. This yolk-/sup 58/Co-B12 test with its ability to detect malabsorption of food-B12 may be considered as an addition to the first part of the Schilling test.

• OSTI ID: 7133750
• Journal Information: Am. J. Clin. Pathol.; (United States), Vol. 80:4
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
VITAMIN B-12
INTESTINAL ABSORPTION
COBALT 57
DUAL-ISOTOPE SUBTRACTION TECHNIQUE
FOOD
INGESTION
LABELLED COMPOUNDS
MAN
MEASURING METHODS
TRACER TECHNIQUES
ABSORPTION
ANIMALS
BETA DECAY RADIOISOTOPES
COBALT ISOTOPES
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
HEMATINICS
HEMATOLOGIC AGENTS
INTAKE
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
PRIMATES
RADIOISOTOPES
UPTAKE
VERTEBRATES
VITAMIN B GROUP
VITAMINS
551001* - Physiological Systems- Tracer Techniques