Specific, transient suppression of the immune response by HGG tolerant spleen cells. II. Effector cells and target cells. [Mice]
The experiments presented here were performed to determine the nature of the effector cell(s) and the target cell(s) involved in this system of suppression of the immune response. Treatment of cells from tolerant animals with anti-thymocyte serum and complement to remove thymus-derived (T) cells completely abrogated suppressive activity. Removal of adherent cells from tolerant spleen cells by passage over glass wool columns resulted in partial loss of the suppression. The inhibitory activity of the suppressor cells was resistant to 900 R irradiation regardless of whether the tolerant spleen cells were irradiated before or after adoptive transfer. The cellular target(s) for the suppressor cells was examined by using lipopolysaccharide (LPS) as an alternative source of helper activity for the response to HGG. LPS, injected at the time of the initial antigenic challenge of mice that had been reconstituted with tolerant and normal spleen cells, prevented the expression of suppression against bone marrow-derived (B) cells. However, when LPS was presented only at the time of the secondary antigenic challenge, it was unable to overcome suppression of the immune response of reconstituted recipients. Thus, LPS could produce a state where the B cells were resistant to suppression, but LPS could not rescue the responsiveness of B cells once the cells in the reconstituted recipient had been suppressed. In addition, the immune responses to both the hapten dinitrophenol (DNP) and the carrier (HGG) were suppressed when recipients of tolerant and normal spleen cells were challenged with DNP/sub 6/HGG. This indicates that T helper cells are also a target for suppression. The results presented in this paper are discussed in relation to a possible mechanism of suppression which proposes that suppressive activity represents the induction of tolerance in immunologically competent cells by HGG which is closely associated with the tolerant spleen cells.
- Research Organization:
- Scripps Clinic and Research Foundation, LaJolla, CA
- OSTI ID:
- 7133093
- Journal Information:
- J. Immunol.; (United States), Vol. 117:4
- Country of Publication:
- United States
- Language:
- English
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DINITROPHENOL
BIOLOGICAL EFFECTS
GLOBULINS-GAMMA
IMMUNE REACTIONS
BIOLOGICAL RADIATION EFFECTS
IMMUNOSUPPRESSION
LIPOPOLYSACCHARIDES
SPLEEN CELLS
BONE MARROW CELLS
GAMMA RADIATION
LETHAL IRRADIATION
MICE
ANIMAL CELLS
ANIMALS
AROMATICS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
GLOBULINS
HYDROXY COMPOUNDS
IONIZING RADIATIONS
IRRADIATION
LIPIDS
MAMMALS
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOLS
POLYSACCHARIDES
PROTEINS
RADIATION EFFECTS
RADIATIONS
RODENTS
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals