New approaches to the design and synthesis of protein-based radiopharmaceuticals with application to the radiolabelling of antibodies

Thesis/Dissertation · Tue Jan 01 00:00:00 EST 1985

OSTI ID:7128680

Layne, W W

A new method of radiolabeling proteins has been explored that involves the coupling of a metal chelating agent, diethylenetriaminepentaacetic acid (DTPA), to proteins in aqueous solution through the use of the DTPA bisanhydride. Each derivatized protein was then radiolabeled with an appropriate carrier-free metallic radionuclide. The proteins examined in this investigation were human serum albumin, human and canine fibrinogen, bovine and human nonspecific gamma globulin, and antibodies to carcinoembryonic antigen (CEA). The two different anti-CEA antibodies chosen for this study were affinity-purified baboon anti-human CEA and monoclonal murine anti-human CEA. The radionuclides were gallium-67, indium-111, and yttrium-90. With /sup 111/In, the method was shown to successfully radiolabel each of the model proteins without significantly altering their in vitro or in vivo behavior.

• Research Organization: Northeastern Univ., Boston, MA (USA)
• OSTI ID: 7128680
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
ANTIBODIES
LABELLING
PROTEINS
RADIOPHARMACEUTICALS
CHEMICAL PREPARATION
ALBUMINS
APES
BLOOD SERUM
CATTLE
DTPA
FIBRINOGEN
GALLIUM 67
GLOBULINS-GAMMA
INDIUM 111
MICE
MONOCLONAL ANTIBODIES
RABBITS
YTTRIUM 90
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
CARBOXYLIC ACIDS
CHELATING AGENTS
COAGULANTS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GALLIUM ISOTOPES
GLOBULINS
HEMATOLOGIC AGENTS
HEMOSTATICS
HOURS LIVING RADIOISOTOPES
INDIUM ISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
RADIOISOTOPES
RADIOPROTECTIVE SUBSTANCES
RODENTS
RUMINANTS
SYNTHESIS
VERTEBRATES
YTTRIUM ISOTOPES
400700* - Radiochemistry & Nuclear Chemistry