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Title: Angiogenin activates phospholipase C and elicits a rapid incorporation of fatty acid into cholesterol esters in vascular smooth muscle cells

Abstract

Angiogenin activates the phosphoinositide-specific phospholipase C (PLC) in cultured rat aortic smooth muscle cells to yield a transient (30 s) peak of 1,2-diacylglycerol (DG) and inositol trisphosphate. Within 1 min, the DG level falls below that of the control and remains so for at least 20 min. A transient increase in monoacylglycerol indicates that depletion of DG may be the consequence of hydrolysis by DG lipase. In addition to these changes in second messengers, a rapid increase in incorporating of radiolabeled tracer into cellular cholesterol esters is observed. Stimulated cholesterol ester labeling is inhibited by preincubation with either the DG lipase inhibitor RHC 80267 or the acyl coenzyme A:cholesterol acyltransferase inhibitor Sandoz 58035. Cells prelabeled with ({sup 3}H)arachidonate show a sustained increase in labeling of cholesterol esters following exposure to angiogenin. In contrast, cells prelabeled with ({sup 3}H)oleate show only a transient elevation that returns to the basal level by 5 min. This suggests initial cholesterol esterification by oleate followed by arachidonate that is released by stimulation of the PLC/DG lipase pathway.

Authors:
;  [1]
  1. (Harvard Medical School, Boston, MA (USA))
Publication Date:
OSTI Identifier:
7118504
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemistry; (USA); Journal Volume: 29:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CHOLESTEROL; ESTERIFICATION; LIPASES; ENZYME INDUCTION; PROTEINS; BIOCHEMISTRY; ANIMAL CELLS; AORTA; ARACHIDONIC ACID; BLOOD VESSELS; MUSCLES; OLEIC ACID; PHOSPHOLIPIDS; RATS; TRITIUM COMPOUNDS; ANIMALS; ARTERIES; BODY; CARBOXYLESTERASES; CARBOXYLIC ACIDS; CARDIOVASCULAR SYSTEM; CHEMICAL REACTIONS; CHEMISTRY; ENZYMES; ESTERASES; ESTERS; GENE REGULATION; HYDROGEN COMPOUNDS; HYDROLASES; HYDROXY COMPOUNDS; LIPIDS; MAMMALS; MONOCARBOXYLIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; ORGANS; RODENTS; STEROIDS; STEROLS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Moore, F., and Riordan, J.F. Angiogenin activates phospholipase C and elicits a rapid incorporation of fatty acid into cholesterol esters in vascular smooth muscle cells. United States: N. p., 1990. Web. doi:10.1021/bi00453a031.
Moore, F., & Riordan, J.F. Angiogenin activates phospholipase C and elicits a rapid incorporation of fatty acid into cholesterol esters in vascular smooth muscle cells. United States. doi:10.1021/bi00453a031.
Moore, F., and Riordan, J.F. Tue . "Angiogenin activates phospholipase C and elicits a rapid incorporation of fatty acid into cholesterol esters in vascular smooth muscle cells". United States. doi:10.1021/bi00453a031.
@article{osti_7118504,
title = {Angiogenin activates phospholipase C and elicits a rapid incorporation of fatty acid into cholesterol esters in vascular smooth muscle cells},
author = {Moore, F. and Riordan, J.F.},
abstractNote = {Angiogenin activates the phosphoinositide-specific phospholipase C (PLC) in cultured rat aortic smooth muscle cells to yield a transient (30 s) peak of 1,2-diacylglycerol (DG) and inositol trisphosphate. Within 1 min, the DG level falls below that of the control and remains so for at least 20 min. A transient increase in monoacylglycerol indicates that depletion of DG may be the consequence of hydrolysis by DG lipase. In addition to these changes in second messengers, a rapid increase in incorporating of radiolabeled tracer into cellular cholesterol esters is observed. Stimulated cholesterol ester labeling is inhibited by preincubation with either the DG lipase inhibitor RHC 80267 or the acyl coenzyme A:cholesterol acyltransferase inhibitor Sandoz 58035. Cells prelabeled with ({sup 3}H)arachidonate show a sustained increase in labeling of cholesterol esters following exposure to angiogenin. In contrast, cells prelabeled with ({sup 3}H)oleate show only a transient elevation that returns to the basal level by 5 min. This suggests initial cholesterol esterification by oleate followed by arachidonate that is released by stimulation of the PLC/DG lipase pathway.},
doi = {10.1021/bi00453a031},
journal = {Biochemistry; (USA)},
number = ,
volume = 29:1,
place = {United States},
year = {Tue Jan 09 00:00:00 EST 1990},
month = {Tue Jan 09 00:00:00 EST 1990}
}