skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Suppression of lymphocyte proliferation by marijuana components is related to cell number and cell source

Abstract

Conflicting reports have appeared concerning the effect of marijuana components on immune responsiveness. The authors have observed that the effect of cannabinoids on lymphocyte proliferation varied with both the concentration of the drug and the mitogen used. They now report that at a constant concentration of drug, the cannabinoid effect varied from no effect to suppression depending upon the number of cells in culture and the organ source of the cells. Dispersed cell suspensions of mouse lymph node, spleen, and thymus were prepared and cultured at varying cell numbers with either delta-9-tetrahydrocannabinol or 11-hydroxy-delta-9-tetrahydrocannabinol and various mitogens. Lymphocyte proliferation was analyzed by /sup 3/H-thymidine incorporation. T-lymphocyte mitogen responses in cultures containing high cell numbers were unaffected by the cannabinoids but as cell numbers were reduced a suppression of the response was observed. Furthermore, thymus cells were considerably more susceptible to cannabinoid suppression than cells from either lymph node or spleen. These results suggest that certain lymphocyte subpopulations are more sensitive to cannabinoid suppression and that in addition to drug concentration other variables such as cell number and cell source must be considered when analyzing cannabinoid effects.

Authors:
; ; ;
Publication Date:
Research Org.:
Univ. of South Florida School of Medicine, Tampa
OSTI Identifier:
7117926
Alternate Identifier(s):
OSTI ID: 7117926
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA
Resource Type:
Journal Article
Resource Relation:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States); Journal Volume: 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; LYMPHOCYTES; CELL PROLIFERATION; MARIHUANA; BIOLOGICAL EFFECTS; CELL CULTURES; IMMUNE REACTIONS; LYMPH NODES; MICE; MITOGENS; SPLEEN; THYMIDINE; THYMUS; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMAL CELLS; ANIMALS; AZINES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CONNECTIVE TISSUE CELLS; HERBS; HETEROCYCLIC COMPOUNDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; LEUKOCYTES; LYMPHATIC SYSTEM; MAMMALS; MATERIALS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PLANTS; PYRIMIDINES; RIBOSIDES; RODENTS; SOMATIC CELLS; VERTEBRATES 560300* -- Chemicals Metabolism & Toxicology; 550201 -- Biochemistry-- Tracer Techniques

Citation Formats

Klein, T., Pross, S., Newton, C., and Friedman, H.. Suppression of lymphocyte proliferation by marijuana components is related to cell number and cell source. United States: N. p., 1986. Web.
Klein, T., Pross, S., Newton, C., & Friedman, H.. Suppression of lymphocyte proliferation by marijuana components is related to cell number and cell source. United States.
Klein, T., Pross, S., Newton, C., and Friedman, H.. Wed . "Suppression of lymphocyte proliferation by marijuana components is related to cell number and cell source". United States.
@article{osti_7117926,
title = {Suppression of lymphocyte proliferation by marijuana components is related to cell number and cell source},
author = {Klein, T. and Pross, S. and Newton, C. and Friedman, H.},
abstractNote = {Conflicting reports have appeared concerning the effect of marijuana components on immune responsiveness. The authors have observed that the effect of cannabinoids on lymphocyte proliferation varied with both the concentration of the drug and the mitogen used. They now report that at a constant concentration of drug, the cannabinoid effect varied from no effect to suppression depending upon the number of cells in culture and the organ source of the cells. Dispersed cell suspensions of mouse lymph node, spleen, and thymus were prepared and cultured at varying cell numbers with either delta-9-tetrahydrocannabinol or 11-hydroxy-delta-9-tetrahydrocannabinol and various mitogens. Lymphocyte proliferation was analyzed by /sup 3/H-thymidine incorporation. T-lymphocyte mitogen responses in cultures containing high cell numbers were unaffected by the cannabinoids but as cell numbers were reduced a suppression of the response was observed. Furthermore, thymus cells were considerably more susceptible to cannabinoid suppression than cells from either lymph node or spleen. These results suggest that certain lymphocyte subpopulations are more sensitive to cannabinoid suppression and that in addition to drug concentration other variables such as cell number and cell source must be considered when analyzing cannabinoid effects.},
doi = {},
journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:4,
place = {United States},
year = {Wed Mar 05 00:00:00 EST 1986},
month = {Wed Mar 05 00:00:00 EST 1986}
}