Electrochemical conversion of carbon dioxide to methanol with the assistance of formate dehydrogenase and methanol dehydrogenase as biocatalysts

Journal Article · Wed Jun 15 00:00:00 EDT 1994 · Journal of the American Chemical Society; (United States)

DOI:https://doi.org/10.1021/ja00091a056· OSTI ID:7110207

Kuwabata, Susumu; Tsuda, Ryo; Yoneyama, Hiroshi ^[1]

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1. Osaka Univ. (Japan)

Electrolysis at potentials between -0.7 and -0.9 V vs SCE of carbon dioxide-saturated phosphate buffer solutions (pH7) containing formate dehydrogenase (FDH) and either methyl viologen (MV[sup 2+]) or pyrroloquinolinequinone (PQQ) as an electron mediator yielded formate with current efficiencies as high as 90%. The enzyme was durable as long as the electrolysis was carried out in the dark. Electrolysis of phosphate buffer solutions containing sodium formate in the presence of methanol dehydrogenase (MDH) and MV[sup 2+] at -0.7 V vs SCE yielded formaldehyde if the concentration of the enzyme used was low, whereas both formaldehyde and methanol were produced for relatively high concentrations of the enzyme where the methanol production began to occur when the formaldehyde produced accumulated. The use of PQQ in place of MV[sup 2+] as the electron mediator exclusively produced methanol alone after some induction period in the electrolysis. On the basis of these results, successful attempts have been made to reduce carbon dioxide to methanol with cooperative assistance of FDH and MDH in the presence of PQQ as the electron mediator. The role of enzyme and mediator in these reduction processes is discussed in detail. 34 refs., 10 figs., 2 tabs.

• OSTI ID: 7110207
• Journal Information: Journal of the American Chemical Society; (United States), Vol. 116:12; ISSN 0002-7863
• Country of Publication: United States
• Language: English

Related Subjects

10 SYNTHETIC FUELS
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
CARBON DIOXIDE
ELECTROLYSIS
FORMALDEHYDE
SYNTHESIS
METHANOL
BIPYRIDINES
BUFFERS
COMPILED DATA
ENZYMES
INFRARED SPECTRA
OXIDOREDUCTASES
PH VALUE
ALCOHOLS
ALDEHYDES
AZINES
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
DATA
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
LYSIS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PROTEINS
PYRIDINES
SPECTRA
100200* - Synthetic Fuels- Production- (1990-)
400102 - Chemical & Spectral Procedures
400400 - Electrochemistry
550200 - Biochemistry