Actions of mammalian insulin on a Neurospora variant: morphology, growth and binding
Molecules resembling mammalian insulin have previously been detected in lower eukaryotes by others, but no function was found. The authors have found that the cell wall-deficient slime mutant of Neurospora crassa, when cultured in a defined, nutrient-rich medium, responds to mammalian insulin (10/sup -10/ M). In transition from the late logarithmic to early stationary phases of growth (18-24 hours), insulin treated cells were larger, rounder and more uniform in shape, and the number of cells with characteristic projections (tails) was reduced 5-fold. Insulin treated cell cultures also contained 15-30% more protein and showed significantly greater viability for an extended period, as measured by trypan blue dye exclusion. Bovine and porcine insulin were both active, as was human insulin produced by recombinant techniques. Binding of insulin to N. crassa slime cells showed properties similar to that of many mammalian cell types: high affinity, reversibility, and a curvilinear Scatchard plot. Even at the low temperature (4/sup 0/C) of these experiments, however, degradation of radiolabeled insulin was high (ca. 30%). The long-term effects of mammalian insulin on N. crassa may thus be mediated through a receptor as in higher organisms.
- Research Organization:
- UMDNJ-Rutgers Medical School, Piscataway
- OSTI ID:
- 7108402
- Report Number(s):
- CONF-8606151-; TRN: 86-039116
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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INSULIN
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL EFFECTS
NEUROSPORA
GROWTH
VIABILITY
AFFINITY
CATTLE
MAN
MOLECULES
MORPHOLOGY
MUTANTS
RADIOISOTOPES
SWINE
TRACER TECHNIQUES
TRYPAN BLUE
AMINES
ANIMALS
AROMATICS
AZO COMPOUNDS
AZO DYES
DOMESTIC ANIMALS
DYES
FUNGI
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
NAPHTHOLS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PEPTIDE HORMONES
PHENOLS
PLANTS
PRIMATES
REACTION KINETICS
RUMINANTS
SULFONIC ACIDS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques