Study of the transfer RNAs coded by T2, T4, and T6 bacteriophages
T2,T4, and T6 bacteriophage tRNAs coding for arginine, leucine, proline, isoleucine, and glycine were isolated under conditions of short term and long term infection of Escherchia coli B cells. The corresponding phage tRNA species were examined for sequence homology by RNA.DNA hybridization analysis and by their relative behavior on reversed phase chromatography. The results indicate that all three T-even phages code for similar tRNA species; however, some tRNA species are homologous, others are not, and not all of the same tRNA species are coded by each bacteriophage. Reversed phase chromatography showed the presence of isoacceptor tRNAs for each phage aminoacyl-tRNA species. Pulse-chase experiments for (/sup 32/P)tRNA/sup Gly/ suggest that the multiple isoacceptor species observed derive from the intracellular modification of a single tRNA/sup Gly/ gene product.
- Research Organization:
- Univ. of Chicago
- OSTI ID:
- 7094370
- Journal Information:
- J. Biol. Chem.; (United States), Vol. 252:14
- Country of Publication:
- United States
- Language:
- English
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