skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Serotonin storage pools in basophil leukemia and mast cells: characterization of two types of serotonin binding protein and radioautographic analysis of the intracellular distribution of (/sup 3/H)serotonin

Abstract

The binding of serotonin to protein(s) derived from rat basophil leukemia (RBL) cells and mast cells was studied. Two types of serotonin binding protein in RBL cells was found. These proteins differed from one another in molecular weight and eluted in separate peaks from sephadex G-200 columns. Peak I protein (KD = 1.9 x 10/sup -6/ M) was a glycoprotein that bound to concanavalin A (Con A); Peak II protein (KD/sub 1/ = 4.5 x 10/sup -/8 M; KD/sub 2/ = 3.9 x 10/sup -6/ M) did not bind to Con A. Moreover, binding of (/sup 3/H)serotonin to protein of Peak I was sensitive to inhibition by reserpine, while binding of (/sup 3/H)serotonin to protein of Peak II resisted inhibition by that drug. Other differences between the two types of binding protein were found, the most significant of which was the far more vigorous conditions of homogenization required to extract Peak I than Peak II protein. Electron microscope radioautographic analysis of the intracellular distribution of (/sup 3/H) serotonin taken up in vitro by RBL cells or in vivo by murine mast cells indicated that essentially all of the labeled amine was located in cytoplasmic granules.No evidence for a pool inmore » the cytosol was found and all granules were capable of becoming labeled. The presence of two types of intracellular serotonin binding proteins in these cells may indicate that there are two intracellular storage compartments for the amine. Both may be intragranular, but Peak I protein may be associated with the granular membrane while Peak II protein may be more free within the granular core. Different storage proteins may help to explain the differential release of amines from mast cell granules.« less

Authors:
 [1]; ; ;
  1. (New York Psychiatric Inst., New York)
Publication Date:
OSTI Identifier:
7089995
Resource Type:
Journal Article
Journal Name:
J. Cell Biol.; (United States)
Additional Journal Information:
Journal Volume: 93:3
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PROTEINS; BINDING ENERGY; SEROTONIN; BIOCHEMICAL REACTION KINETICS; AUTORADIOGRAPHY; BASOPHILS; CONCANAVALIN; ELECTRON MICROSCOPY; LABELLED COMPOUNDS; LEUKEMIA; MAST CELLS; RATS; TRITIUM COMPOUNDS; AGGLUTININS; AMINES; ANIMAL CELLS; ANIMALS; ANTIBODIES; AUTONOMIC NERVOUS SYSTEM AGENTS; AZOLES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DISEASES; DRUGS; ENERGY; HEMAGGLUTININS; HEMIC DISEASES; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INDOLES; KINETICS; LEUKOCYTES; MAMMALS; MATERIALS; MICROSCOPY; NEOPLASMS; NEUROREGULATORS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PYRROLES; RADIOPROTECTIVE SUBSTANCES; REACTION KINETICS; RODENTS; SOMATIC CELLS; SYMPATHOMIMETICS; TRYPTAMINES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Tamir, H., Theoharides, T.C., Gershon, M.D., and Askenase, P.W. Serotonin storage pools in basophil leukemia and mast cells: characterization of two types of serotonin binding protein and radioautographic analysis of the intracellular distribution of (/sup 3/H)serotonin. United States: N. p., 1982. Web. doi:10.1083/jcb.93.3.638.
Tamir, H., Theoharides, T.C., Gershon, M.D., & Askenase, P.W. Serotonin storage pools in basophil leukemia and mast cells: characterization of two types of serotonin binding protein and radioautographic analysis of the intracellular distribution of (/sup 3/H)serotonin. United States. doi:10.1083/jcb.93.3.638.
Tamir, H., Theoharides, T.C., Gershon, M.D., and Askenase, P.W. Tue . "Serotonin storage pools in basophil leukemia and mast cells: characterization of two types of serotonin binding protein and radioautographic analysis of the intracellular distribution of (/sup 3/H)serotonin". United States. doi:10.1083/jcb.93.3.638.
@article{osti_7089995,
title = {Serotonin storage pools in basophil leukemia and mast cells: characterization of two types of serotonin binding protein and radioautographic analysis of the intracellular distribution of (/sup 3/H)serotonin},
author = {Tamir, H. and Theoharides, T.C. and Gershon, M.D. and Askenase, P.W.},
abstractNote = {The binding of serotonin to protein(s) derived from rat basophil leukemia (RBL) cells and mast cells was studied. Two types of serotonin binding protein in RBL cells was found. These proteins differed from one another in molecular weight and eluted in separate peaks from sephadex G-200 columns. Peak I protein (KD = 1.9 x 10/sup -6/ M) was a glycoprotein that bound to concanavalin A (Con A); Peak II protein (KD/sub 1/ = 4.5 x 10/sup -/8 M; KD/sub 2/ = 3.9 x 10/sup -6/ M) did not bind to Con A. Moreover, binding of (/sup 3/H)serotonin to protein of Peak I was sensitive to inhibition by reserpine, while binding of (/sup 3/H)serotonin to protein of Peak II resisted inhibition by that drug. Other differences between the two types of binding protein were found, the most significant of which was the far more vigorous conditions of homogenization required to extract Peak I than Peak II protein. Electron microscope radioautographic analysis of the intracellular distribution of (/sup 3/H) serotonin taken up in vitro by RBL cells or in vivo by murine mast cells indicated that essentially all of the labeled amine was located in cytoplasmic granules.No evidence for a pool in the cytosol was found and all granules were capable of becoming labeled. The presence of two types of intracellular serotonin binding proteins in these cells may indicate that there are two intracellular storage compartments for the amine. Both may be intragranular, but Peak I protein may be associated with the granular membrane while Peak II protein may be more free within the granular core. Different storage proteins may help to explain the differential release of amines from mast cell granules.},
doi = {10.1083/jcb.93.3.638},
journal = {J. Cell Biol.; (United States)},
number = ,
volume = 93:3,
place = {United States},
year = {1982},
month = {6}
}