Ulcerative colitis and Crohn's disease tissue cytotoxins

Journal Article · Tue Jun 01 00:00:00 EDT 1982 · Gastroenterology; (United States)

OSTI ID:7087082

McLaren, L C; Gitnick, G

Bowel-wall tissue filtrates from patients with inflammatory bowel disease produce cytopathic effects in tissue culture. The cytopathic effects inducers have been reported to have the characteristics of a small RNA virus. Clostridium difficile toxin also produces cytopathic effects and has been found in the stools of patients with Crohn's disease and ulcerative colitis. The present study concerns the further characterization of the cytopathic inducers in tissues of inflammatory bowel disease patients. It was found that they are nonsedimentable at 148,000 g for 2 h and resistant to inactivation by UV light. They are proteins that are distinct from C. difficile toxin and are unique cytotoxins which are associated with the early cytopathic effects observed in Riff-free chick embryo and rabbit ileum cell cultures. These results suggest that the early cytopathic effects previously described are not produced by a virus. They do not explain the delayed cytopathic effects seen in rabbit ileum or WI-38 cells.

• Research Organization: Department of Microbiology, University of New Mexico, School of Medicine, Albuquerque
• OSTI ID: 7087082
• Journal Information: Gastroenterology; (United States), Vol. 82:6
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL CULTURES
QUANTITATIVE CHEMICAL ANALYSIS
TOXINS
BIOLOGICAL EFFECTS
BIOLOGICAL WASTES
CHICKENS
CLOSTRIDIUM
COMPARATIVE EVALUATIONS
EMBRYOS
INTESTINES
PATHOLOGY
PATIENTS
PROTEINS
ULCERS
ULTRAFILTRATION
ULTRAVIOLET RADIATION
ANIMALS
ANTIGENS
BACTERIA
BIOLOGICAL MATERIALS
BIRDS
BODY
CHEMICAL ANALYSIS
DIGESTIVE SYSTEM
ELECTROMAGNETIC RADIATION
FILTRATION
FOWL
GASTROINTESTINAL TRACT
MATERIALS
MICROORGANISMS
ORGANIC COMPOUNDS
ORGANS
PATHOLOGICAL CHANGES
RADIATIONS
SEPARATION PROCESSES
TOXIC MATERIALS
VERTEBRATES
WASTES
550900* - Pathology