Variation in the sensitivity of the mouse spermatogonial stem cell population to fission neutron irradiation during the cycle of the seminiferous epithelium

Journal Article · Mon Dec 01 00:00:00 EST 1986 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576916· OSTI ID:7084308

van Beek, M E; Davids, J A; de Rooij, D G

Dose-response studies of the radiosensitivity of spermatogonial stem cells in various epithelial stages after irradiation with graded doses of fission neutrons of 1 MeV mean energy were carried out in the Cpb-N mouse. These studies on the stem cell population in stages IX-XI yielded simple exponential lines characterized by an average D0 value of 0.76 +/- 0.02 Gy. In the subsequent epithelial stages XII-III, a significantly lower D0 value of 0.55 +/- 0.02 Gy was found. In contrast to the curves obtained for stem cells in stages IX-III, the curves obtained in stages IV-VIII indicated the presence of a mixture of radioresistant and radiosensitive stem cells. In stage VII, almost no radioresistant stem cells appeared to be present and a D0 value for the radiosensitive stem cells of 0.22 +/- 0.01 Gy was derived. Previously, data were obtained on the size of colonies (in number of spermatogonia) derived from surviving stem cells. Combining these data with data from the newly obtained dose-response curves yielded the number of stem cells, per stage and with the specific radiosensitivities, present in the control epithelium. In stages IX-XI, there are approximately 6 stem cells per 1000 Sertoli cells with a radiosensitivity characterized by a D0 of 0.76 Gy, which corresponds to one-third of the As population in these stages. (The As spermatogonia are presumed to be the stem cells of spermatogenesis.) IN stages XII-III, there are approximately 12 stem cells per 1000 Sertoli cells with a radiosensitivity characterized by a D0 of 0.55 Gy, which roughly equals the number of A single spermatogonia in these stages. These calculations could not be made for stages IV-VIII since no simple exponential lines were obtained for these stages.

• Research Organization: State Univ., Medical School, Utrecht, The Netherlands
• OSTI ID: 7084308
• Journal Information: Radiat. Res.; (United States), Vol. 3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
STEM CELLS
RADIOSENSITIVITY
CELL DIVISION
DOSE-RESPONSE RELATIONSHIPS
EPITHELIUM
FISSION NEUTRONS
GENETIC VARIABILITY
MICE
RADIATION DOSES
SPERMATOGONIA
SPERMATOZOA
TESTES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BARYONS
BIOLOGICAL VARIABILITY
BODY
DOSES
ELEMENTARY PARTICLES
FERMIONS
GAMETES
GERM CELLS
GONADS
HADRONS
MALE GENITALS
MAMMALS
NEUTRONS
NUCLEONS
ORGANS
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture