Evidence that auxin-induced growth of soybean hypocotyls involves proton excretion

Journal Article · Mon Sep 01 00:00:00 EDT 1980 · Plant Physiol.; (United States)

DOI:https://doi.org/10.1104/pp.66.3.433· OSTI ID:7079186

Rayle, D L ^[1]; Cleland, R E

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1. San Diego State Univ., CA

The role of H/sup +/ excretion in auxin-induced growth of soybean hypocotyl tissues has been investigated, using tissues whose cuticle was rendered permeable to protons or buffers by scarification (scrubbing). Indoleacetic acid induces both elongation and H/sup +/ excretion after a lag of 10 to 12 minutes. Cycloheximide inhibits growth and causes the tissues to remove protons from the medium. Neutral buffers (pH 7.0) inhibit auxin-induced growth of scrubbed but not intact sections; the inhibition increases as the buffers strength is increased. Both live and frozen-thawed sections, in the absence of auxin, extend in response to exogenously supplied protons. Fusicoccin induces both elongation and H/sup +/ excretion at rates greater than does auxin. These results indicate that H/sup +/ excretion is involved in the initiation of auxin-induced elongation in soybean hypocotyl tissue.

• DOE Contract Number: EY-76-S-06-2225
• OSTI ID: 7079186
• Journal Information: Plant Physiol.; (United States), Vol. 66:3
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
59 BASIC BIOLOGICAL SCIENCES
AUXINS
BIOCHEMICAL REACTION KINETICS
LEGUMINOSAE
METABOLISM
PLANT TISSUES
QUANTITATIVE CHEMICAL ANALYSIS
ACETIC ACID
BOTANY
BUFFERS
CYCLOHEXIMIDE
ELONGATION
ENZYME ACTIVITY
HYDROGEN IONS 1 PLUS
INDOLES
INHIBITION
MORPHOLOGY
PERMEABILITY
PH VALUE
PLANT GROWTH
PLANT STEMS
PROTONS
TIME DEPENDENCE
ULTRASTRUCTURAL CHANGES
ANTIBIOTICS
AZOLES
BARYONS
BIOLOGY
CARBOXYLIC ACIDS
CATIONS
CHARGED PARTICLES
CHEMICAL ANALYSIS
DRUGS
ELEMENTARY PARTICLES
FERMIONS
FUNGICIDES
GROWTH
HADRONS
HETEROCYCLIC COMPOUNDS
HYDROGEN IONS
IONS
KINETICS
MONOCARBOXYLIC ACIDS
MORPHOLOGICAL CHANGES
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PESTICIDES
PLANT GROWTH REGULATORS
PLANTS
PYRROLES
REACTION KINETICS
553000* - Agriculture & Food Technology
550200 - Biochemistry
550800 - Morphology