Production of ethanol from biopolymers by anaerobic, thermophilic, and extreme thermophilic bacteria. III. Thermoanaerobacter ethanolicus JW200 and its mutants in batch cultures and resting cell experiments
Several thermophilic and extreme thermophilic anaerobic bacteria can utilize hemicellulose (xylan polymer) from birch- and beechwood directly. Thermoanaerobacter ethanolicus JW200 exhibited the highest ethanol formation, although the extracellular xylanase and xylosidase activities were very low. All bacteria rapidly utilized xylose before the polymers were utilized at a lower rate. With resting cell suspensions of T. ethanolicus and its mutants, the ethanol formation rates were as high as 60 mmol (2.76 g) and 30 mmol (1.3 g) ethanol per liter per hour from glucose and xylose, respectively. After 1 hour the ethanol productions in the concentrated cell suspensions were linear for over 10 hours from glucose or xylose; however, with soluble starch (DE 10) the rates were increasing with time. From these experiments it is concluded that the contiuous culture experiments with hemicellulosic material and/or starch have to be performed with recycling of the cells and the extracellular enzymes. 25 references, 6 figures, 3 tables.
- Research Organization:
- Univ. of Georgia, Athens
- DOE Contract Number:
- AS09-79ER10499
- OSTI ID:
- 7077526
- Report Number(s):
- CONF-830567-
- Journal Information:
- Biotechnol. Bioeng. Symp.; (United States), Vol. 13; Conference: 5. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN, USA, 10 May 1983
- Country of Publication:
- United States
- Language:
- English
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Production of ethanol from biopolymers by anaerobic, thermophilic, and extreme thermophilic bacteria. I. Regulation of carbohydrate utilization in mutants of Thermoanaerobacter ethanolicus
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
COMPARATIVE EVALUATIONS
ETHANOL
PRODUCTION
GLUCOSE
FERMENTATION
HEMICELLULOSE
STARCH
XYLANS
XYLOSE
BEECH TREES
BIOCHEMICAL REACTION KINETICS
BIRCHES
CLOSTRIDIUM THERMOCELLUM
CLOSTRIDIUM THERMOSACCHAROLYTICUM
DECOMPOSITION
GROWTH
MUTANTS
ALCOHOLS
ALDEHYDES
BIOCONVERSION
CARBOHYDRATES
CHEMICAL REACTIONS
CLOSTRIDIUM
HEXOSES
HYDROXY COMPOUNDS
KINETICS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PENTOSES
PLANTS
POLYSACCHARIDES
REACTION KINETICS
REAGENTS
SACCHARIDES
TREES
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