Tandem mass spectrometry of highly charged cytochrome c molecular ions produced by electrospray ionization
- Pacific Northwest Lab., Richland, WA (USA)
The dissociation of multiply charged molecular ions of cytochrome c (MW 12,360 daltons) formed by electrospray ionization (ESI) has been studied by use of tandem (triple quadrupole) mass spectrometry. For the selected experimental conditions both metastable and collision-induced-dissociation (CID) processes are observed for the 14+ to 19+ protonated molecular ions, while lower charge state spectra (9+ to 12+) are dominated by charge-transfer and charge-stripping processes. For higher charge states two classes of dissociation processes are evident. The first involves dissociation (both metastable and CID) from higher internal energy (and apparently thermalized) molecular ions and yields distinctive fragments in the m/z region about the molecular ion. The second occurs for higher collision energies and yields a very large number of low intensity products across a wide m/z range. The collisional excitation gained in the ESI/vacuum interface and the slow dissociation rates anticipated for large molecular ions appear important to these observations.
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 7071255
- Journal Information:
- Journal of Physical Chemistry; (USA), Vol. 93:13; ISSN 0022-3654
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
CYTOCHROMES
MASS SPECTROSCOPY
DATA ANALYSIS
EXPERIMENTAL DATA
MEASURING INSTRUMENTS
MEASURING METHODS
MOLECULAR IONS
CHARGED PARTICLES
DATA
INFORMATION
IONS
NUMERICAL DATA
ORGANIC COMPOUNDS
PIGMENTS
PROTEINS
SPECTROSCOPY
400201* - Chemical & Physicochemical Properties
400000 - Chemistry
550000 - Biomedical Sciences
Basic Studies