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Title: Effect of somatostatin on nonesterified fatty acid levels modifies glucose homeostasis during fasting

Abstract

In the 7-days fasted conscious dog, unlike the postabsorptive conscious dog, somatostatin infusion results in decreased levels of nonesterified fatty acids (NEFA) and increased glucose utilization (R{sub d}) even when insulin and glucagon levels are held constant. The aim of this study was to determine whether NEFA replacement in such animals would prevent the increase in R{sub d}. In each of three protocols there was an 80-min tracer equilibration period, a 40-min basal period, and a 3-h test period. During the test period in the first protocol saline was infused, in the second protocol somatostatin was infused along with intraportal replacement amounts of insulin and glucagon (hormone replacement), while in the third protocol somatostatin plus the pancreatic hormones were infused with concurrent heparin plus Intralipid infusion. Glucose turnover was assessed using (3-{sup 3}H)glucose. The peripheral levels of insulin, glucagon, and glucose were similar and constant in all three protocols; however, during somatostatin infusion, exogenous glucose infusion was necessary to maintain euglycemia. The NEFA level was constant during saline infusion and decreased in the hormone replacement protocol. In the hormone replacement plus NEFA protocol, the NEFA level did not change during the first 90-min period and then increased during the secondmore » 90-min period. After a prolonged fast in the dog, (1) somatostatin directly or indirectly inhibits adipose tissue NEFA release and causes a decrease in the plasma NEFA level, and (2) this decrease in the NEFA level causes an increase in R{sub d}.« less

Authors:
; ;  [1]
  1. (Vanderbilt Univ. School of Medicine, Nashville, TN (USA))
Publication Date:
OSTI Identifier:
7068324
Resource Type:
Journal Article
Resource Relation:
Journal Name: American Journal of Physiology; (USA); Journal Volume: 253:4
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; CARBOXYLIC ACIDS; BIOCHEMISTRY; GLUCAGON; RADIOIMMUNOASSAY; SOMATOSTATIN; BIOLOGICAL EFFECTS; DOGS; GLUCOSE; HOMEOSTASIS; IODINE 125; TRITIUM COMPOUNDS; ALDEHYDES; ANIMALS; BETA DECAY RADIOISOTOPES; BIOASSAY; CARBOHYDRATES; CHEMISTRY; DAYS LIVING RADIOISOTOPES; DIAGNOSTIC TECHNIQUES; ELECTRON CAPTURE RADIOISOTOPES; HEXOSES; HORMONES; HYDROGEN COMPOUNDS; IMMUNOASSAY; IMMUNOLOGY; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MONOSACCHARIDES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; PEPTIDE HORMONES; PEPTIDES; POLYPEPTIDES; PROTEINS; RADIOASSAY; RADIOIMMUNODETECTION; RADIOIMMUNOLOGY; RADIOISOTOPES; SACCHARIDES; TRACER TECHNIQUES; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Hendrick, G.K., Frizzell, R.T., and Cherrington, A.D. Effect of somatostatin on nonesterified fatty acid levels modifies glucose homeostasis during fasting. United States: N. p., 1987. Web.
Hendrick, G.K., Frizzell, R.T., & Cherrington, A.D. Effect of somatostatin on nonesterified fatty acid levels modifies glucose homeostasis during fasting. United States.
Hendrick, G.K., Frizzell, R.T., and Cherrington, A.D. Thu . "Effect of somatostatin on nonesterified fatty acid levels modifies glucose homeostasis during fasting". United States.
@article{osti_7068324,
title = {Effect of somatostatin on nonesterified fatty acid levels modifies glucose homeostasis during fasting},
author = {Hendrick, G.K. and Frizzell, R.T. and Cherrington, A.D.},
abstractNote = {In the 7-days fasted conscious dog, unlike the postabsorptive conscious dog, somatostatin infusion results in decreased levels of nonesterified fatty acids (NEFA) and increased glucose utilization (R{sub d}) even when insulin and glucagon levels are held constant. The aim of this study was to determine whether NEFA replacement in such animals would prevent the increase in R{sub d}. In each of three protocols there was an 80-min tracer equilibration period, a 40-min basal period, and a 3-h test period. During the test period in the first protocol saline was infused, in the second protocol somatostatin was infused along with intraportal replacement amounts of insulin and glucagon (hormone replacement), while in the third protocol somatostatin plus the pancreatic hormones were infused with concurrent heparin plus Intralipid infusion. Glucose turnover was assessed using (3-{sup 3}H)glucose. The peripheral levels of insulin, glucagon, and glucose were similar and constant in all three protocols; however, during somatostatin infusion, exogenous glucose infusion was necessary to maintain euglycemia. The NEFA level was constant during saline infusion and decreased in the hormone replacement protocol. In the hormone replacement plus NEFA protocol, the NEFA level did not change during the first 90-min period and then increased during the second 90-min period. After a prolonged fast in the dog, (1) somatostatin directly or indirectly inhibits adipose tissue NEFA release and causes a decrease in the plasma NEFA level, and (2) this decrease in the NEFA level causes an increase in R{sub d}.},
doi = {},
journal = {American Journal of Physiology; (USA)},
number = ,
volume = 253:4,
place = {United States},
year = {Thu Oct 01 00:00:00 EDT 1987},
month = {Thu Oct 01 00:00:00 EDT 1987}
}