/sup 31/P NMR characterization of graded traumatic brain injury in rats
Irreversible tissue injury following central nervous system trauma is believed to result from both mechanical disruption at the time of primary insult, and more delayed autodestructive processes. These delayed events are associated with various biochemical changes, including alterations in phosphate energy metabolism and intracellular pH. Using /sup 31/P NMR, we have monitored the changes in phosphorus energy metabolism and intracellular pH in a single hemisphere of the rat brain over an 8-h period following graded, traumatic, fluid percussion-induced brain injury. Following trauma the ratio of phosphocreatine to inorganic phosphate (PCr/Pi) declined in each injury group. This decline was transitory with low injury (1.0 +/- 0.5 atm), biphasic with moderate (2.1 +/- 0.4 atm) and high (3.9 +/- 0.9 atm) injury, and sustained following severe injury (5.9 +/- 0.7 atm). The initial PCr/Pi decline in the moderate and high injury groups was associated with intracellular acidosis; however, the second decline occurred in the absence of any pH changes. Alterations in ATP occurred only in severely injured animals and such changes were associated with marked acidosis and 100% mortality rate. After 4h, the posttraumatic PCr/Pi ratio correlated linearly with the severity of injury. We suggest that a reduced posttraumatic PCr/Pi ratio may be indicative of altered mitochondrial energy production and may predict a reduced capacity of the cell to recover from traumatic injury.
- Research Organization:
- Univ. of California, San Francisco (USA)
- OSTI ID:
- 7067829
- Journal Information:
- Magnet. Reson. Med.; (United States), Vol. 6:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BRAIN
INJURIES
PHOSPHATES
METABOLISM
QUANTITY RATIO
PHOSPHOCREATINE
BLOOD PRESSURE
NUCLEAR MAGNETIC RESONANCE
PH VALUE
PHOSPHORUS 31
RATS
AMINO ACIDS
ANIMALS
BODY
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
RESONANCE
RODENTS
STABLE ISOTOPES
VERTEBRATES
550901* - Pathology- Tracer Techniques