Microbial detection of mutagenic nitro-organic compounds in filtrates of coal fly ash

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Environ. Mutagen.; (United States)

DOI:https://doi.org/10.1002/em.2860040307· OSTI ID:7065413

Wei, C ^[1]; Raabe, O G; Rosenblatt, L S

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1. Univ. of California, Davis

The presence of mutagenic nitro-organic compounds on coal fly ash was indicated by the greatly reduced microbial mutagenicity of the ash filtrates with nitroreductase-deficient strains of Salmonella typhimurium compared to their corresponding parental strains. Addition of the liver S-9 microsomal enzyme preparation significantly increased the mutagenic activities of the ash extracts. Extracts of fly ash mutagens were prepared with horse serum, dimethyl sulfoxide, or azeotropic benzene/methanol mixture. The data were normalized to net revertants per 10/sup 8/ Salmonella typhimurium cells per milligram of ash used. This normalization procedure is essential for interpretation of comparative results. Both four-way and three-way analyses of variance were used to simultaneously evaluate the differences between solvent extracts, fly ash mutagen, S-9 activation, and nitroreductase-deficient strains and their parental strains. Of the three extraction systems tested, benzene/methanol azeotropic mixture was generally found to have the highest extraction power, and horse serum was the lowest. The results show that overall 87.5% (+/- 1.8 SE) of the mutagenic activity of the fly ash was associated with nitro-organic compounds.

• OSTI ID: 7065413
• Journal Information: Environ. Mutagen.; (United States), Vol. 4:3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
02 PETROLEUM
FLY ASH
BIOLOGICAL EFFECTS
ORGANIC NITROGEN COMPOUNDS
MUTAGEN SCREENING
BIOLOGICAL INDICATORS
COAL
METABOLIC ACTIVATION
SALMONELLA
AEROSOL WASTES
ASHES
BACTERIA
CARBONACEOUS MATERIALS
ENERGY SOURCES
FOSSIL FUELS
FUELS
MATERIALS
MICROORGANISMS
ORGANIC COMPOUNDS
RESIDUES
SCREENING
WASTES
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)
020900 - Petroleum- Environmental Aspects