In vitro lymphocyte proliferation induced by radio-frequency electromagnetic radiation under isothermal conditions
- Virginia Commonwealth Univ., Richmond (USA)
Whole human blood was exposed or sham-exposed in vitro for 2 h to 27 or 2,450 MHz radio-frequency electromagnetic (RF) radiation under isothermal conditions (i.e., 37 +/- 0.2{degrees}C). Immediately after exposure, mononuclear cells were separated from blood by Ficoll density-gradient centrifugation and cultured for 3 days at 37 degrees C with or without mitogenic stimulation by phytohemagglutinin (PHA). Lymphocyte proliferation was assayed at the end of the culture period by 6 h of pulse labeling with 3H-thymidine (3H-TdR). Exposure to radiation at either frequency at specific absorption rates (SARs) below 50 W/kg resulted in a dose-dependent, statistically significant increase of 3H-TdR uptake in PHA-activated or unstimulated lymphocytes. Exposure at 50 W/kg or higher suppressed 3H-TdR uptake relative to that of sham-exposed cells. There were no detectable effects of RF radiation on lymphocyte morphology or viability. Notwithstanding the characteristic temperature dependence of lymphocyte activation in vitro, the isothermal exposure conditions of this study warrant the conclusion that the biphasic, dose-dependent effects of the radiation on lymphocyte proliferation were not dependent on heating.
- OSTI ID:
- 7061536
- Journal Information:
- Bioelectromagnetics (New York); (USA), Vol. 11:1; ISSN 0197-8462
- Country of Publication:
- United States
- Language:
- English
Similar Records
Glioma proliferation modulated in vitro by isothermal radiofrequency radiation exposure
Effect of microwaves (2450-MHz) on the immune system in mice: studies of nucleic acid and protein synthesis
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
LYMPHOCYTES
CELL PROLIFERATION
RADIOWAVE RADIATION
BIOLOGICAL EFFECTS
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC FIELDS
IN VITRO
MAN
MONOCYTES
TEMPERATURE DEPENDENCE
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
LEUKOCYTES
MAMMALS
MATERIALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RADIATIONS
RIBOSIDES
SOMATIC CELLS
VERTEBRATES
560400* - Other Environmental Pollutant Effects
550301 - Cytology- Tracer Techniques