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Title: Cellulose digestion in Monochamus marmorator Kby. (coleoptera: Cerambycidae): role of acquired fungal enzymes

Abstract

Larvae of the balsam fir sawyer, Monochamus marmorator Kby. (Coleoptera, Cerambycidae), contain midgut digestive enzymes active against hemicellulose and cellulose. Cellulases from larvae fed on balsam fir wood infected with the fungus, Trichoderma harzianum Rifai (Deuteromycetes, Moniliales, Moniliaceae), were found to be identical to those of the cellulase complex produced by this fungus when compared using chromatography, electrophoresis, and isofocusing. When larvae are maintained on a fungusfree diet, their midgut fluids lack cellulolytic activity, and they are unable to digest cellulose. Cellulolytic capacity can be restored by feeding the larvae wood permeated by fungi. We conclude that the enzymes which enable M. marmorator larvae to digest cellulose are not produced by the larvae. Instead, the larvae acquire the capacity to digest cellulose by ingesting active fungal cellulases while feeding in fungus-infected wood.

Authors:
;
Publication Date:
Research Org.:
Univ of Michigan, Am Arbor, MI
OSTI Identifier:
7058239
Alternate Identifier(s):
OSTI ID: 7058239
Resource Type:
Journal Article
Journal Name:
J. Chem. Ecol.; (United States)
Additional Journal Information:
Journal Volume: 12:5
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CELLULASE; CELLULOLYTIC ACTIVITY; CELLULOSE; DIGESTION; FUNGI; HEMICELLULOSE; LARVAE; DIET; RADIOCHEMICAL ANALYSIS; CARBON 14; CHROMATOGRAPHY; ELECTROPHORESIS; INGESTION; LABELLED COMPOUNDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOHYDRATES; CARBON ISOTOPES; CHEMICAL ANALYSIS; ENZYME ACTIVITY; ENZYMES; EVEN-EVEN NUCLEI; GLYCOSYL HYDROLASES; HYDROLASES; INTAKE; ISOTOPES; LIGHT NUCLEI; NUCLEI; O-GLYCOSYL HYDROLASES; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; QUANTITATIVE CHEMICAL ANALYSIS; RADIOISOTOPES; SACCHARIDES; SEPARATION PROCESSES; YEARS LIVING RADIOISOTOPES 550501* -- Metabolism-- Tracer Techniques

Citation Formats

Kukol, J.J., and Martin, M.M. Cellulose digestion in Monochamus marmorator Kby. (coleoptera: Cerambycidae): role of acquired fungal enzymes. United States: N. p., 1986. Web.
Kukol, J.J., & Martin, M.M. Cellulose digestion in Monochamus marmorator Kby. (coleoptera: Cerambycidae): role of acquired fungal enzymes. United States.
Kukol, J.J., and Martin, M.M. Thu . "Cellulose digestion in Monochamus marmorator Kby. (coleoptera: Cerambycidae): role of acquired fungal enzymes". United States.
@article{osti_7058239,
title = {Cellulose digestion in Monochamus marmorator Kby. (coleoptera: Cerambycidae): role of acquired fungal enzymes},
author = {Kukol, J.J. and Martin, M.M.},
abstractNote = {Larvae of the balsam fir sawyer, Monochamus marmorator Kby. (Coleoptera, Cerambycidae), contain midgut digestive enzymes active against hemicellulose and cellulose. Cellulases from larvae fed on balsam fir wood infected with the fungus, Trichoderma harzianum Rifai (Deuteromycetes, Moniliales, Moniliaceae), were found to be identical to those of the cellulase complex produced by this fungus when compared using chromatography, electrophoresis, and isofocusing. When larvae are maintained on a fungusfree diet, their midgut fluids lack cellulolytic activity, and they are unable to digest cellulose. Cellulolytic capacity can be restored by feeding the larvae wood permeated by fungi. We conclude that the enzymes which enable M. marmorator larvae to digest cellulose are not produced by the larvae. Instead, the larvae acquire the capacity to digest cellulose by ingesting active fungal cellulases while feeding in fungus-infected wood.},
doi = {},
journal = {J. Chem. Ecol.; (United States)},
number = ,
volume = 12:5,
place = {United States},
year = {1986},
month = {5}
}