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Title: Microbial enhanced oil recovery research. [Peptides]

Abstract

The surface active lipopeptide produced by Bacillus licheniformis JF-2 was isolated to near apparent homogeneity. NMR experiments revealed that this compound consists of a heptapeptide with an amino acid sequence similar to surfactin and a heterogeneous fatty acid consisting of the normal-, anteiso-, and iso- branched isomers. The surface activity of the B. licheniformis JF-2 surfactant was shown to depend on the presence of fermentation products and is strongly affected by the pH. Under conditions of optimal salinity and pH the interfacial tension against decane was 6 [times] 10[sup 3] mN/m which is one of the lowest values ever obtained with a microbial surfactant. Microbial compounds which exhibit particularly high surface activity are classified as biosurfactants. Microbial biosurfactants include a wide variety of surface and interfacially active compounds, such as glycolipids, lipopeptides polysaccharideprotein complexes, phospholipids, fatty acids and neutral lipids. Biosurfactants are easily biodegradable and thus are particularly suited for environmental applications such as bioremediation and the dispersion of oil spills. Bacillus licheniformis strain JF-2 has been shown to be able to grow and produce a very effective biosurfactant under both aerobic and anaerobic conditions and in the presence of high salt concentrations. The production of biosurfactants in anaerobic, highmore » salt environments is potentially important for a variety of in situ applications such as microbial enhanced oil recovery. As a first step towards evaluating the commercial utility of the B. licheniformis JF-2 surfactant, we isolated t-he active. compound from the culture supernatant, characterized its chemical structure and investigated its phase behavior. We found that the surface activity of the surfactant is strongly dependent on the pH of the aqueous. phase. This may be important for the biological function of the surfactant and is of interest for several applications in surfactancy.« less

Authors:
;  [1]
  1. Texas Univ., Austin, TX (United States)
Publication Date:
Research Org.:
Texas Univ., Austin, TX (United States). Dept. of Petroleum Engineering
Sponsoring Org.:
USDOE; USDOE, Washington, DC (United States)
OSTI Identifier:
7053191
Report Number(s):
DOE/BC/14445-T3
ON: DE93008388
DOE Contract Number:  
FG22-89BC14445
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
02 PETROLEUM; BACILLUS LICHENIFORMIS; GROWTH; MICROBIAL EOR; BENCH-SCALE EXPERIMENTS; SURFACTANTS; BIOSYNTHESIS; STRUCTURAL CHEMICAL ANALYSIS; ANAEROBIC CONDITIONS; CARBON 13; EXPERIMENTAL DATA; INFRARED SPECTRA; INTERFACES; NMR SPECTRA; OIL WELLS; PH VALUE; PROGRESS REPORT; SURFACE TENSION; BACILLUS; BACTERIA; CARBON ISOTOPES; DATA; DOCUMENT TYPES; ENHANCED RECOVERY; EVEN-ODD NUCLEI; INFORMATION; ISOTOPES; LIGHT NUCLEI; MICROORGANISMS; NUCLEI; NUMERICAL DATA; SPECTRA; STABLE ISOTOPES; SURFACE PROPERTIES; SYNTHESIS; WELLS; 020300* - Petroleum- Drilling & Production

Citation Formats

Sharma, M M, and Georgiou, G. Microbial enhanced oil recovery research. [Peptides]. United States: N. p., 1992. Web. doi:10.2172/7053191.
Sharma, M M, & Georgiou, G. Microbial enhanced oil recovery research. [Peptides]. United States. https://doi.org/10.2172/7053191
Sharma, M M, and Georgiou, G. 1992. "Microbial enhanced oil recovery research. [Peptides]". United States. https://doi.org/10.2172/7053191. https://www.osti.gov/servlets/purl/7053191.
@article{osti_7053191,
title = {Microbial enhanced oil recovery research. [Peptides]},
author = {Sharma, M M and Georgiou, G},
abstractNote = {The surface active lipopeptide produced by Bacillus licheniformis JF-2 was isolated to near apparent homogeneity. NMR experiments revealed that this compound consists of a heptapeptide with an amino acid sequence similar to surfactin and a heterogeneous fatty acid consisting of the normal-, anteiso-, and iso- branched isomers. The surface activity of the B. licheniformis JF-2 surfactant was shown to depend on the presence of fermentation products and is strongly affected by the pH. Under conditions of optimal salinity and pH the interfacial tension against decane was 6 [times] 10[sup 3] mN/m which is one of the lowest values ever obtained with a microbial surfactant. Microbial compounds which exhibit particularly high surface activity are classified as biosurfactants. Microbial biosurfactants include a wide variety of surface and interfacially active compounds, such as glycolipids, lipopeptides polysaccharideprotein complexes, phospholipids, fatty acids and neutral lipids. Biosurfactants are easily biodegradable and thus are particularly suited for environmental applications such as bioremediation and the dispersion of oil spills. Bacillus licheniformis strain JF-2 has been shown to be able to grow and produce a very effective biosurfactant under both aerobic and anaerobic conditions and in the presence of high salt concentrations. The production of biosurfactants in anaerobic, high salt environments is potentially important for a variety of in situ applications such as microbial enhanced oil recovery. As a first step towards evaluating the commercial utility of the B. licheniformis JF-2 surfactant, we isolated t-he active. compound from the culture supernatant, characterized its chemical structure and investigated its phase behavior. We found that the surface activity of the surfactant is strongly dependent on the pH of the aqueous. phase. This may be important for the biological function of the surfactant and is of interest for several applications in surfactancy.},
doi = {10.2172/7053191},
url = {https://www.osti.gov/biblio/7053191}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1992},
month = {Wed Jan 01 00:00:00 EST 1992}
}