Ontogeny of basic fibroblast growth factor binding sites in mouse ocular tissues

Journal Article · Tue May 01 00:00:00 EDT 1990 · Experimental Cell Research; (USA)

DOI:https://doi.org/10.1016/0014-4827(90)90280-N· OSTI ID:7045542

Fayein, N A; Courtois, Y; Jeanny, J C ^[1]

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1. INSERM U. 118, Paris (France)

Basic fibroblast growth factor (bFGF) binding to ocular tissues has been studied by autoradiographical and biochemical approaches directly performed on sections during mouse embryonic and postnatal development. Frozen sections of embryos (9 to 18 days), newborns, and adults (1 day to 6 months) were incubated with iodinated bFGF. One specific FGF binding site (KD = 2.5 nM) is colocalized with heparan sulfate proteoglycans of the basement membranes and is heparitinase sensitive. It first appears at Day 9 around the neural tube, the optic vesicles, and below the head ectoderm and by Day 14 of embryonic development is found in all basement membranes of the eye. At Day 16, very intensely labeled patches appear, corresponding to mast cells which have been characterized by metachromatic staining of their heparin-rich granulations with toluidine blue. In addition to the latter binding, we have also observed a general diffuse distribution of silver grains on all tissues and preferentially in the ecto- and neuroectodermic tissues. From Days 17-18, there is heterogeneous labeling inside the retina, localized in the pigmented epithelium and in three different layers colocalized with the inner and outer plexiform layers and with the inner segments of the photoreceptors. This binding is heparitinase resistant but N-glycanase sensitive and may represent a second specific binding site corresponding to cellular FGF receptors (KD = 280 pM). Both types of binding patterns observed suggest a significant role for bFGF in eye development and physiology.

• OSTI ID: 7045542
• Journal Information: Experimental Cell Research; (USA), Vol. 188:1; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
EYES
ONTOGENESIS
GROWTH FACTORS
RECEPTORS
BIOLOGICAL LOCALIZATION
AUTORADIOGRAPHY
BIOCHEMICAL REACTION KINETICS
EPITHELIUM
FIBROBLASTS
HISTOLOGICAL TECHNIQUES
HYDROLASES
IODINE ISOTOPES
MEMBRANE PROTEINS
MICE
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BODY
BODY AREAS
CONNECTIVE TISSUE CELLS
ENZYMES
FACE
HEAD
ISOTOPES
KINETICS
MAMMALS
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
REACTION KINETICS
RODENTS
SENSE ORGANS
SOMATIC CELLS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques