Characterization of amino acid metabolism by cultured rat kidney cells: Study with sup 15 N

Journal Article · Tue Dec 01 00:00:00 EST 1987 · American Journal of Physiology; (USA)

OSTI ID:7027241

Nissim, I; States, B; Yudkoff, M; Segal, S ^[1]

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1. Children's Hospital of Philadelphia, PA (USA) Univ. of Pennsylvania School of Medicine, Philadelphia (USA)

The present study evaluates the metabolism of glutamine and glutamate by normal rat kidney (NRK) cells. The major aim was to evaluate the effect of acute acidosis on the metabolism of amino acid and ammonia formation by cultured NRK cells. Experiments at either pH 7.0 or 7.4 were conducted with phosphate-buffered saline supplemented with either 1 mM (5-{sup 15}N)glutamine, (2-{sup 15}N)glutamine, or ({sup 15}N)glutamate. Incubation with either glutamine or glutamate as a precursor showed that production of ammonia and glucose was increased significantly at pH 7.0 vs 7.4. In experiments with (5-{sup 15}N)glutamine, the authors found that {approximately}57 and 43% of ammonia N was derived from 5-N of glutamine at pH 7.4 and 7.0, respectively. Three major metabolic pathways of (2-{sup 15}N)glutamine or ({sup 15}N)glutamate disposal were identified: (1) transamination reactions involving the pH-independent formation of ({sup 15}N) aspartate and ({sup 15}N)alanine; (2) the synthesis of (6-{sup 15}NH{sub 2})adenine nucleotide, a process more active at pH 7.4 vs. 7.0; and (3) glutamine synthesis from ({sup 15}N)glutamate, especially at pH 7.4. The data indicate that NRK cells in culture consume glutamine and glutamate and generate ammonia and various amino acids, depending on the H{sup +} concentration in the media. The studies suggest that these cell lines may provide a useful model for studying various aspects of the effect of pH on rat renal ammoniagenesis.

• OSTI ID: 7027241
• Journal Information: American Journal of Physiology; (USA), Vol. 253:6; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
METABOLISM
GLUTAMIC ACID
BIOSYNTHESIS
GLUTAMINE
NITROGEN 15
ALANINES
AMMONIA
ANIMAL CELLS
ASPARTIC ACID
GAS CHROMATOGRAPHY
GLUCOSE
KIDNEYS
LABELLED COMPOUNDS
MASS SPECTROSCOPY
PH VALUE
PROTONS
RATS
ALDEHYDES
AMIDES
ANIMALS
BARYONS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CHROMATOGRAPHY
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HEXOSES
HYDRIDES
HYDROGEN COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MONOSACCHARIDES
NITROGEN COMPOUNDS
NITROGEN HYDRIDES
NITROGEN ISOTOPES
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
RODENTS
SACCHARIDES
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques